xSPECT Bone® (xB) is a new reconstruction algorithm developed by Siemens® in bone hybrid imaging (SPECT/CT). A CT-based tissue segmentation is incorporated into SPECT reconstruction to provide SPECT images with bone anatomy appearance. The objectives of this study were to assess xB/CT reconstruction diagnostic reliability and accuracy in comparison with Flash 3D® (F3D)/CT in clinical routine. Two hundred thirteen consecutive patients referred to the Brest Nuclear Medicine Department for non-oncological bone diseases were evaluated retrospectively. Two hundred seven SPECT/CT were included. All SPECT/CT were independently interpreted by two nuclear medicine physicians (a junior and a senior expert) with xB/CT then with F3D/CT three months later. Inter-observer agreement (IOA) and diagnostic confidence were determined using McNemar test, and unweighted Kappa coefficient. The study objectives were then re-assessed for validation through > 18 months of clinical and paraclinical follow-up.
No statistically significant differences between IOA xB and IOA F3D were found (p = 0.532). Agreement for xB after categorical classification of the diagnoses was high (κxB = 0.89 [95% CI 0.84 –0.93]) but without statistically significant difference F3D (κ F3D = 0.90 [95% CI 0.86 – 0.94]). Thirty-one (14.9%) inter-reconstruction diagnostic discrepancies were observed of which 21 (10.1%) were classified as major. The follow-up confirmed the diagnosis of F3D in 10 cases, xB in 6 cases and was non-contributory in 5 cases.
xB reconstruction algorithm was found reliable, providing high interobserver agreement and similar diagnostic confidence to F3D reconstruction in clinical routine.
xSPECT Bone® (xB) is a new iterative reconstruction algorithm developed by Siemens® for bone single photon emission computed tomography (SPECT). Unlike classic SPECT reconstructions, xB uses ordered subset conjugate gradient minimization algorithm (OSCGM). Its originality consists of constraining counts in computed tomography (CT) based on bone segmentation (Fig. 1) and providing a quantitative reconstruction [1, 2].
This innovation, like the progress of image acquisition and reconstruction, could convey a higher diagnostic confidence through an enhanced bone uptake location. Studies have reported early planar images with good sensitivity yet poor specificity. The latter was improved when using SPECT reconstructions with negative predictive value while maintaining an excellent sensitivity [3,4,5]. Moreover, the use of CT improved the specificity of SPECT , particularly concerning small lesions. Besides, physical limitations such as attenuation or Compton scattering have also benefited from corrections integrated directly into reconstruction algorithms, leading to less artifacts and shorter reconstruction time. Then, the “side-by-side” display of SPECT and CT images (SPECT + CT) was replaced by fused SPECT/CT images [6,7,8,9,10]. In this manner, Römer et al. were able to identify 90% of SPECT findings classified as indeterminate . These authors also indicated that exact matching of functional and anatomic data may be necessary, especially for imaging of small anatomic structures.
That said, taking into account patient’s clinical data should also be regarded as a mainstay in enhancement of overall diagnostic confidence of scintigraphy.
In the end, in non-oncological context, the objective of both clinician and health care provider is to reduce additional imaging that could delay patient management, increase stress, and induce additional irradiation.
The objectives of this study were:
First, to evaluate the reliability of xB/CT bone reconstruction in comparison with that of Flash 3D® (F3D)/CT.
Second, to evaluate the diagnostic confidence of xB/CT compared with that of F3D/CT for non-oncological painful bone diseases according to the recommendations of good practice of the European Association of Nuclear Medicine .
A retrospective study was conducted on 213 non-oncological patients referred for a bone scintigraphy at the Nuclear Medicine Department of Brest University Hospital from March to September 2014. Seven patients were excluded (four due to a poor image fusion between SPECT and CT related to important movements, one for whom the SPECT/CT was not retrieved from PACS (picture archiving and communication system), another for whom the field of view of the CT was too small, and finally one who declined to participate in the study). All patients were given verbal information before the exams that their data could be used for future scientific research and gave their written consent.
The SPECT/CT of 206 patients was analyzed (70 male and 136 female) with 13 patients younger than 18 years old. Their mean age ± SD was 53.2 ± 18.8.
Two hundred seven SPECT/CT were included for 206 patients (2 SPECT/CT performed for the same patient). The anatomical areas explored are summarized in Table 1.
SPECT/CT data were acquired between 2 and 4 h after the intravenous injection of approximately 9 MBq/kg of 99mTcDPD (TECEOS®, CIS bio-international, 91112 Gif-Sur-Yvette, France) on a Symbia Intevo T6 dual-headed gamma camera (Siemens® SAS Medical Solutions, Munich, Germany) equipped with a low-energy high-resolution parallel-hole collimator. The energy window was set at 15%, centered on the photon energy peak of 99mTc (140 keV).
The SPECT acquisition protocol was as follows: 60 frames per detector head, each with duration of 10 s, were acquired in step-and-shoot mode over 360° with non-circular orbit. Acquisition matrix was 256 × 256 to allow xB reconstruction.
The CT acquisition was performed immediately after the SPECT acquisition as follows: the image matrix size was 512 × 512, with a tube voltage of 110 kV for the extremities and 130 kV out of the extremities; automatic exposure control system (CARE Dose4D) with 90 quality reference mAs; a pitch of 1.05 for the extremities and 1.0 out of the extremities; a slice thickness of 5 mm for attenuation correction (AC), 1.25 mm for the extremities, and 3 mm out of the extremities; and a field of view of 30 cm for the extremities and 50 cm out of the extremities including the knees. FBP reconstructions were used with a B31s filter for AC, IRIS iterative reconstructions with i30s and i80s filters for analysis.
The SPECT/CT acquisition for the wrist and hand was performed on prone position.
Reconstructions xB and F3D
The goal of iterative reconstruction is to find the best estimated slice that, when projected in multiple directions, is as close to acquired projections as possible. xSPECT is based on OSCGM algorithm, and xB is a variant of xSPECT® that considers that almost all the 99mTc-DPD is localized in bones. First, CT is re-sampled to xSPECT® resolution (2562). CT data are then segmented in five increasing DPD uptake probability areas (air, adipose tissue, soft tissue, spongy bone, cortical bone). Those probabilities are incorporated in the xB algorithm, which constrains the reconstructed data in high uptake probability area, especially bones. To speed up the computation, ordered subsets can also be used .
The xB reconstruction was first performed with 36 iterations and 1 subset, a 256 × 256 matrix that leads to a 2.4-mm pixel and a 10-mm full width at the half maximum (FWHM) Gaussian post-filter. Then, an undersampling from 2562 to 1282 was performed on the projections in order to perform F3D reconstruction, with 8 iterations, 15 subsets, and a 12-mm FWHM Gaussian post-filter.
Co-registered CT, SPECT, and SPECT/CT images were visualized with a commercially available 3D volume fusion tool (Syngo.via®, Siemens Healthcare). The 3D images were displayed as 2D orthogonal (axial, coronal, and sagittal, automatically generated by multiplanar reformatting (MPR) from the axial slices) and maximum intensity projections (MIP) on two screens through a custom display, allowing spatial synchronization through a triangulation pattern. The look-up table of the SPECT/CT images was “Warm Metal.”
Interpretation and analysis of data
Retrospectively, all bone scans were independently interpreted by two nuclear medicine physicians (one senior physician (experience of 15 years) and one junior physician (experience of 3 years)) with the xB/CT reconstruction and then 3 months later in order to obtain a blind interpretation of the first one with F3D/CT reconstruction. Choosing a junior and a senior physician would allow assessment of the diagnostic confidence reliability and accuracy in bone reconstruction, whatever the reader’s experiences. Each bone scintigraphy was interpreted by simultaneous analysis of the SPECT, CT, and SPECT/CT reconstructions. The interpretation was made with the knowledge of the clinical context, i.e., our clinical routine.
The diagnoses were classified into five categories: 1—normal scintigraphy, 2—arthritis, 3—periarticular disease, 4—fracture or tumor pathology, and 5—complex regional pain syndrome. The diagnoses are summarized in Table 2.
First, the interpretation discrepancies between the two physicians were identified within xB and within F3D. In case of diagnostic discrepancy between the two physicians, the diagnosis was made after consensus. In a second step, the diagnostic differences between xB and F3D reconstructions after harmonization within each reconstruction were identified. They were classified as major (if the diagnosis and the treatment were different) or minor (if they were irrelevant and did not lead to any therapeutic modification).
Clinical and paraclinical follow-up
For all included patients, the follow-up was carried out either by consulting the medical file or by calling the referring physician and/or the patient directly. Due to the retrospective nature of our study, the referring physician had already received the F3D reconstruction report. The clinical and paraclinical follow-up was carried out over 18 months after scintigraphy of the last patient. Of the 206 patients, 204 were followed up. Two patients were lost to follow-up. Patients’ additional data are summarized in Table 3.
The diagnostic differences (major or minor) between xB and F3D reconstructions were compared with the clinical and paraclinical follow-up, considered as the reference standard in our study.
The comparison of the IOA by reconstruction was performed according to two distinct statistical methods: raw diagnoses were compared with a McNemar test and diagnostic categories were compared with an unweighted kappa coefficient (according to the five categories mentioned above).
The retrospective nature of the study did not allow us to have a reference standard independent from the index test. Indeed, for situations in which a difference in diagnosis was observed between xB and F3D reconstructions, a simple descriptive comparison with the follow-up was performed.
Inter-observer agreement (IOA) and inter-observer discrepancy (IOD)
Among the 207 SPECT/CT interpreted with xB then with F3D, 23 IOD were observed within F3D without IOD for these same 23 cases with xB, thus representing 11.1% of IOD in the F3D arm. Similarly, 18 IOD were observed within xB without IOD for these same 18 cases with F3D, representing 8.7% of IOD in the xB group. For the remaining 166 examinations, no IOD was found in both xB and F3D (Table 4).
A McNemar test showed no statistically significant difference between IOA xB and IOA F3D (p = 0.532).
Moreover, the unweighted kappa coefficient calculated after categorical classification of the diagnoses was high but did not demonstrate a statistically significant difference between F3D and xB: kappa F3D = 0.90 [95% CI 0.86–0.94] and kappa xB = 0.89 [95% CI 0.84–0.93]. The contingency table of the diagnosis is presented in Table 5, according to the two physicians after categorical classification of the diagnosis.
Thirty-one (14.9%) IRDD were observed out of 207 SPECT/CT, with raw diagnosis or categorical diagnosis. Twenty-one (10.1%) IRDD were classified as major and 10 (4.8%) IRDD as minor.
Among the 21 major IRDD, the follow-up confirmed the diagnosis of F3D in 10 cases and xB in 6 cases and was non-contributory in 5 cases. Of the 16 cases for which follow-up was informative, there were 5 false negatives for F3D and 4 false negatives for xB, 4 false positives for xB but none for F3D and 3 localization errors, 2 for xB and one for F3D. IRDD are described in Table 6.
Forty-one (19.8%) IOD were observed for the 207 SPECT/CT. For the 31 IRDD, 13 IOD (41.9%) were observed. Seven IOD (33.3%) were observed for the 21 major IRDD, and 6 IOD (60%) were observed for the 10 minor IRDD.
Analysis of scintigraphy with bone prosthesis
Twenty-four bone scans concerned an exploration of pain involving joints with prosthetic replacement. Three IOD (12.5%) were identified (one for xB and two for F3D). Only one IRDD was identified. Follow-up concluded to a false negative of xB. No false positive was identified with xB. It should be noted that for four scans with concordant findings xB and F3D, follow-up was contradictory (three false negatives and one false positive results).
The Siemens® xB tomographic image reconstruction is a new way of bone image reconstruction theoretically being suggested to provide better bone contrast, thus high-quality images compared with conventional reconstructions. This hypothesis should be assessed for confirmation. To our knowledge, this study is the first to evaluate the diagnostic reliability and accuracy of this novel reconstruction in routine clinical practice. The study includes a large number of patients and their follow-up and concludes to a high inter-observer agreement and a similar diagnostic confidence as compared with F3D.
A high kappa index for xB (0.89) [95% CI 0.84–0.93] showed a very strong IOA, highlighting the reliability of interpretation, between junior and senior expert readers.
The kappa index obtained according to F3D reconstructions was also high (0.90) with a confidence interval [95% CI 0.86–0.94] without statistically significant differences in inter-observer agreement. The same conclusions were obtained with the McNemar test (p = 0.532). We thus observed equivalent diagnostic confidence between xB and F3D reconstructions.
Thirty-one IRDD (14.9%) were observed among the 207 SPECT/CT. Of the 31 IRDD, 21 were classified as major (10.1%). A diagnosis was made according to the follow-up in most IRDD cases (16/21). With a better spatial resolution to observe smaller SPECT abnormalities and a better bone to soft tissue contrast, xB may theoretically allow increased detection and better visualization of weakly evolving or small abnormalities that could go unnoticed with F3D. However, according to our clinical experience, detecting smaller or weakly evolving abnormalities did not have a major clinical relevance and did not lead to a therapeutic modification.
Indeed, the higher the IRDD proportion, the higher is the IOD percentage (i.e., high IRDD 41.9% vs. low IRDD 19.8%): borderline bone scan abnormalities were most likely interpreted subjectively (i.e., between physicians with different experience) and therefore more likely to induce IOD, with less clinical relevance.
Of the 16 IRDD, 10 diagnoses done by F3D vs. 6 by xB were confirmed through follow-up. This difference was explained in particular by a higher number of false positives for xB, 4 against none for F3D (Fig. 2). However, the number of false negatives was almost equivalent, 5 for F3D and 4 for xB (Fig. 3). Finally, three radiopharmaceutical uptake errors were observed: two in xB and one in F3D. One radiopharmaceutical uptake error in xB was due to patient movements between the SPECT and the CT acquisitions (Fig. 4). These were not detectable on xB/CT fused images alone but were detectable on F3D/CT images. Aberrant xB images due to location uptake errors are easy to identify. However, when the movements are minimal, they can be undetectable and lead to diagnostic error. This suggests the necessity to systematically take a look at the F3D/CT slices in order to control the accurate registration of SPECT and CT slices in xB reconstruction. Nevertheless, the good spatial resolution of xB can ease the reading and thus change the diagnosis. This is illustrated in Fig. 5: a joint disorder was diagnosed between talus and trigonum bones using xB (and confirmed by follow-up) and as a talus contusion using F3D.
It should also be noted that the study was not carried out by comparing only the SPECT reconstructions but rather by comparing the registered images SPECT/CT with knowledge of the clinical context. The use of CT slices and the knowledge of pain mechanism may have an impact on the diagnostic confidence of the scintigraphy. Thereby, Vija et al.  demonstrated significantly higher accuracy of xB used without CT slices compared to F3D. However, the difference was no longer statistically significant between the two reconstructions when fused with the CT slices.
Finally, the striking innovation of the xB reconstruction is the esthetic aspect of SPECT images, which could ease visualization and interpretation of anomalies on MIP images. This combined with clinical and paraclinical findings may enhance patient management and treatment.
Thus, the ease of interpretation provided by xB could bring an added value to the scintigraphic examination given the resultant high-quality images. Most clinicians pay close attention to the images and this often without reading the acquisition report .
Given the technological advancement in bone scintigraphy, clinicians and health care provider’s objectives are to highlight diagnostic confidence thus limiting the use of additional imagery.
All together, we believe that prospective studies are warranted to reach more conclusive results in regard with xB reconstruction reliability and accuracy in bone imaging. This further step can help at reaching robust clinical evidence as well as diagnostic consensus.
Similarly, to repeat this study in a multicentric way would limit the interpretation bias observed in our study. Our diagnostic decisions were not independent given that the junior physician was trained by the senior physician from our nuclear medicine department. Nevertheless, the inter-observer agreement scores are comparable to those observed in the literature (0.87–0.97) [8, 15,16,17,18].
Our study demonstrated that xB reconstruction algorithm was a reliable tool in diagnosis of non-oncological bone diseases, providing high inter-observer agreement and similar diagnostic confidence compared with F3D. Moreover, it may improve SPECT/CT images quality thanks to a striking esthetic aspect. Moreover, the proper registration between SPECT and CT slices needs to be checked systematically in F3D images.
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The principal investigators were OD and SQ. They performed the interpretations of all bone scintigraphy and the redaction of the manuscript and its submission. MG performed the statistical analysis. DB helped us with the technical aspect of the reconstruction algorithm. PYS, RA, ALD-P, PYLR, and PR helped us imagine the design of our study and improve it. ZA revised and wrote the intellectual content of the manuscript and helped at data and result interpretation. All authors read and approved the final manuscript.
At the time of the study, in France, no approval by an ethics committee was required for retrospective studies. All patients gave written informed consent for the scientific use of their data.
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All patients included gave written informed consent that their data could be used for scientific purposes.
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Delcroix, O., Robin, P., Gouillou, M. et al. A new SPECT/CT reconstruction algorithm: reliability and accuracy in clinical routine for non-oncologic bone diseases.
EJNMMI Res8, 14 (2018). https://doi.org/10.1186/s13550-018-0367-7