Microglial depletion and activation: A [11C]PBR28 PET study in nonhuman primates
© The Author(s). 2017
Received: 6 June 2017
Accepted: 11 July 2017
Published: 24 July 2017
The 18-kDa translocator protein (TSPO) is an important target for assessing neuroimmune function in brain with positron-emission tomography (PET) imaging. The goal of this work was to assess two [11C]PBR28 imaging paradigms for measuring dynamic microglia changes in Macaca mulatta.
Dynamic [11C]PBR28 PET imaging data with arterial blood sampling were acquired to quantify TSPO levels as [11C]PBR28 V T. Scans were acquired at three timepoints: baseline, immediately post-drug, and prolonged post-drug.
In one animal, a colony-stimulating factor 1 receptor kinase inhibitor, previously shown to deplete brain microglia, reduced [11C]PBR28 V T in brain by 46 ± 3% from baseline, which recovered after 12 days to 7 ± 5% from baseline. In a different animal, acute lipopolysaccharide administration, shown to activate brain microglia, increased [11C]PBR28 V T in brain by 39 ± 9% from baseline, which recovered after 14 days to −11 ± 3% from baseline.
These studies provide preliminary evidence of complementary paradigms to assess microglia dynamics via in vivo TSPO imaging.
The immune system in the central nervous system is critical to maintaining homeostasis in the brain. Imbalances in the brain’s immune system are linked to a variety of neurological pathologies . Microglia are resident macrophages in the central nervous system that function as critical regulators of neuroimmune function. Microglia are quiescent under normal conditions ; however, microglia become “activated” in response to cytokines or other substances indicating the presence of neuroinflammation. Microglial activation is a necessary repair process, but dysregulated microglial function can cause neuronal dysfunction and cell death . Thus, pharmaceutical therapeutics targeting microglia are of high interest to regulate neuroimmune response in many central nervous system disorders.
The colony-stimulating factor 1 receptor (CSF1R) is a tyrosine kinase of interest for regulating neuroimmune response. The endogenous ligands CSF1 and interleukin-34 (IL-34) act at the CSF1R, altering the activation and proliferation of microglia. Mice born without the Csf1r gene do not develop microglia and rarely survive to adulthood , while extensive treatment of rodents with CSF1R-selective inhibitors eliminated nearly all microglia in the brain . Indeed, CSF1R inhibitors improved behavioral measures in a mouse model of Alzheimer’s disease  and reduced tumor-associated macrophage levels . Moreover, microglia are the only brain cells expressing CSF1R , making drugs targeting CSF1R “microglial selective” in the brain. Assessment of microglia with molecular imaging could provide a critical tool evaluating important physiological effects of CSF1R treatments for psychiatry and neurodegenerative disorders.
Positron-emission tomography (PET) imaging of the 18-kDa translocator protein (TSPO) is a current state-of-the-art technique for the in vivo measurement of neuroinflammation. TSPO primarily is expressed on the outer mitochondrial membrane of microglia and reactive astrocytes, with low expression levels in other neuronal cells . Additionally, TSPO levels are expressed at high levels when microglia are activated . PET imaging with TSPO-specific radioligands such as [11C]PBR28 (N-acetyl-N-(2-[11C]methoxybenzyl)-2-phenoxy-5-pyridinamine) is commonly interpreted as a measure of activated microglia, although astrocytes and other neuronal cells expressing TSPO also contribute to the signal. Recent studies demonstrated that acute administration of Escherichia coli lipopolysaccharide (LPS), which triggers robust microglial activation, increased [11C]PBR28 volumes of distribution (V T) by 30–60% from baseline values in both baboons  and humans , which immunohistochemistry revealed to result almost exclusively in microglia . However, the prolonged effects of acute LPS on TSPO imaging measures are unknown. Moreover, measurements of TSPO levels following microglial depletion have not been performed; such studies would provide a powerful complement to measurement of microglial response. The goal of this preliminary report is to assess two complementary [11C]PBR28 PET imaging paradigms for measuring dynamic changes in neuroinflammation, including microglial depletion with a CSF1R inhibitor and microglial activation via LPS administration, at both acute and prolonged time periods in Macaca mulatta.
Two Macaca mulatta animals (both male, 6–7 years, 11–17 kg) were imaged.
PLX3397 , a selective CSF1R inhibitor (provided by Plexxikon Inc., Berkeley, CA), was made available in the animal’s daily biscuit allotment and in preferred foods at 165 mg/kg/day for seven consecutive days (15–21 days after baseline [11C]PBR28 scan). This dosing regimen robustly reduced brain microglia in rodents in a previous report5. However, not all the food was consumed. Therefore, on the following day (22 days after baseline scan), an additional oral gavage of 165 mg/kg PLX3397 was administered 2 h prior to the second [11C]PBR28 PET scan. A third [11C]PBR28 PET scan was acquired 12 days after the second [11C]PBR28 PET scan to assess recovery of TSPO levels.
LPS (NIH Clinical Center Reference Endotoxin E. coli serotype O:113) was used to induce microglial activation as shown previously [9, 10]. In a second animal, a baseline [11C]PBR28 scan was first acquired. After completion of the baseline scan, 1 ng/kg LPS (the dose given in the previous human imaging study ) was administered as a slow bolus over 1 min. A second [11C]PBR28 scan was acquired, starting 2.5 h after LPS administration to coincide with maximal neuroinflammatory and cytokine response [9, 10], in order to examine the acute effects of LPS on microglial activation. A third [11C]PBR28 PET scan was acquired 14 days after LPS administration to assess prolonged effects of LPS on microglial activation.
[11C]PBR28 was produced at high specific activity (> 120 MBq/nmol) as previously described . Injected PBR28 masses were less than 1.54 nmol (range 0.16–1.54 nmol). At least 2 h before PET scanning, animals were initially anesthetized with ketamine hydrochloride (10 mg/kg, i.m.), and maintained on anesthesia with 1.5–2.5% isoflurane throughout the scan. Two catheters were placed, one in a saphenous vein for radiotracer administration and a second in a radial or femoral artery for blood sampling. Vital signs were continuously monitored for the duration of scanning procedures. PET imaging data were acquired with a Focus 220 (Siemens/CTI, Knoxville, TN). Data acquisition in list-mode began simultaneously with a slow 3-min bolus injection of 177–183 MBq [11C]PBR28, and continued for 120 min. To measure the metabolite-corrected [11C]PBR28 input function and free fraction (f P), arterial blood samples were manually acquired with the same sampling and analysis methods as previously described for nonhuman primates . For region of interest identification, a T1-weighted MR image was acquired separately with a Siemens 3 T Trio scanner using an extremity coil and the following spin echo sequence: [echo time (TE) = 3.34 ms, repetition time (TR) = 2530 ms, flip angle = 7°, thickness = 0.50 mm, field of view = 140 mm]. Non-brain structures were removed using FMRIB’s Brain Extraction Tool.
List-mode PET data were histogrammed into time bins up to 5 min long and reconstructed with Fourier rebinning followed by 2-D filtered back projection with a 0.15-mm−1 Shepp filter. Reconstructed PET images were registered to each animal’s MR image, and the animal’s MR image was then normalized to a MRI atlas using the nonlinear registration algorithm in BioImage Suite 3.01. Time-activity curves were extracted from the caudate, cerebellum, cingulate cortex, frontal cortex, hippocampus, occipital cortex, putamen, temporal cortex, and thalamus. The primary imaging outcome measure was [11C]PBR28 total distribution volume (V T) , calculated using multilinear analysis method (MA1)  with t* = 15 min. Values of V T were estimated for all regions of interest for primary analyses and further estimated at the voxel level to create parameterized maps for visual assessment.
Results and discussion
[11C]PBR28 experimental results
f P (%)
V T (mL/cm3)
V T (mL/cm3)
% Change from BL
V T (mL/cm3)
% Change from BL
V T (mL/cm3)
V T (mL/cm3)
% Change from BL
V T (mL/cm3)
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In contrast, acute administration of 1 ng/mg LPS robustly increased [11C]PBR28 V T by 39 ± 9% (averaged across regions) relative to baseline values (Table 1). Fourteen days later, [11C]PBR28 values were 11 ± 3% lower (averaged across regions) than baseline V T values (Fig. 1b). Taken together, these data demonstrate the potential for two complementary imaging paradigms that assay dynamic changes in TSPO levels in vivo.
The reduction of [11C]PBR28 V T following administration of PLX3397 is consistent with previous reports of microglial depletion throughout the brains of mice following administration of CSF1R inhibitors . While TSPO is expressed in cells other than microglia such as astrocytes and endothelial cells, CSF1R inhibitors are specific to microglia in brain , and astrocyte morphology remained unchanged following CSF1R inhibition in rodents . Therefore, the observed reduction in [11C]PBR28 V T most likely results from depletion of microglia expressing TSPO. Previous TSPO saturation studies in rhesus monkeys demonstrated that over 95% of [11C]PBR28 V T is attributed to specific binding , thus the 46 ± 3% reduction in [11C]PBR28 V T could result from a partial depletion of microglia, a reflection of the astrocyte contribution to baseline V T values, or a combination of the two. The small differences (7 ± 5%) in [11C]PBR28 V T between baseline and 12 days after the course of PLX3397 are consistent with reported recovery of microglial levels after termination of CSF1R inhibition . Our findings here suggest that TSPO imaging with PET could be used to measure microglial depletion via drugs such as CSF1R inhibitors in vivo.
The increase of [11C]PBR28 V T following the administration of 1 ng/kg LPS is also consistent with previously reported increase (30–60%) of [11C]PBR28 V T 3 h following administration of the same LPS dose in humans . Similar results were also reported for baboons, where 29% and 62% increases in [11C]PBR28 V T were reported following 1 and 4 h, respectively, post administration of 0.1 mg/kg LPS . The previous baboon study acquired a further [11C]PBR28 scan 22 h after LPS administration in two animals to examine sub-acute LPS effects. In those scans, [11C]PBR28 V T was reduced by 43% compared to baseline in one animal, whereas [11C]PBR28 V T returned to baseline in the second animal. It is unclear whether prolonged anesthesia may have influenced those results. The present data further suggest that TSPO levels recover by 14 days following LPS-induced activation, as [11C]PBR28 V T values were 11% lower than baseline at this time. While additional studies are needed for confirmation, these data support the hypothesis that TSPO levels recover over an extended time after LPS administration. While the test-retest reproducibility of [11C]PBR28 V T in rhesus monkey has not formally been reported, the test-retest variability in humans at our imaging center is 8% in gray matter regions . Thus the differences in our measured [11C]PBR28 V T values at extended imaging timepoints relative to baseline are likely consistent with test-retest variability.
Previous studies noted that LPS administration reduced [11C]PBR28 concentrations in arterial plasma. Here, a similar effect was observed following LPS administration. A possible explanation for this behavior is increased specific uptake of radiotracer not only in brain but throughout the periphery, reducing parent radiotracer concentrations available in arterial plasma. Following PLX3397 administration, [11C]PBR28 concentrations in arterial plasma increased from baseline by roughly 50%, while tissue concentration in the brain also decreased from baseline by roughly 25%, resulting in a net decrease of 46% in V T. Similarly, this net increase in [11C]PBR28 concentrations in arterial plasma could have resulted from less specific uptake of radiotracer throughout the periphery following chronic PLX3397 administration. In addition, f P changed in the acute scan from both paradigms. Following 1 ng/kg LPS administration, f p increased by 38%, contrasting previous human studies where f P remained unchanged following the same LPS dose . Following CSF1R inhibition with PLX3397, f P decreased from 7.8 to 5.7%; however, V T/f P values were still 27 ± 4% lower than baseline. Importantly, the PLX3397 dosing schedule did not affect blood-brain barrier (BBB) integrity in rodents , thus is it unlikely that the reported V T values were biased by a compromised BBB. To summarize, because administration of PLX3397 or LPS results in complex effects on [11C]PBR28 behavior in both the blood and the tissue, we contend that V T should be used as the outcome measure to fully capture the changes in brain levels of TSPO with this imaging paradigm.
This report is not without caveats. Only one animal was imaged for each of the microglial activation and microglial depletion paradigms. A larger sample size would improve confidence in these findings, yet we believe that the robust findings and within subject design provide preliminary proof of principle for this imaging paradigm. In addition, the interpretation of microglial depletion following PLX3397 administration is inferred from prior rodent studies instead of direct determination using methods such as immunohistochemistry or post mortem protein binding. Finally, the exact dose of PLX3397 received by the animal is not known, as not all the food was consumed. Therefore the timing and oral dose of CSF1R inhibitor for 46 ± 3% reduction of [11C]PBR28 V T in rhesus monkey is unclear.
We present data preliminarily indicating two complementary TSPO imaging paradigms allowing for dynamic in vivo measurements of microglial depletion and microglial activation. These techniques are of great interest for studying neuroinflammation in neurodegenerative and psychiatric disorders, and for the evaluation of therapeutics targeting microglia in the brain.
We thank the staff at the Yale PET Center for their expert technical assistance with this work. This work was supported by NIH grants T32 DA022975, K02 DA031750, and the Veterans Affairs National Center for Post-Traumatic Stress Disorder. PLX3397 was generously provided by Plexxikon, Inc.
This work was supported by NIH grants T32 DA022975, K02 DA031750, and the Veterans Affairs National Center for Post-Traumatic Stress Disorder. PLX3397 was generously provided by Plexxikon, Inc.
BLW, REC, and KPC designed the study. NN oversaw radiolabeling. DH and KF oversaw animal care and imaging data acquisition. ATH, DH, KF, and NN performed data analysis. ATH and KPC drafted the initial manuscript version. All authors contributed to interpretation of data, manuscript revisions, and approval of the final draft.
Ethics approval and consent to participate
All procedures followed institutional guidelines and were approved by the Yale University Institutional Animal Care and Use Committee, in compliance with Animal Research: Reporting in Vivo Experiments guidelines.
Dr. Brian West is an employee of Plexxikon, Inc. All other authors have no competing interests to report.
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- Dantzer R, O'Connor JC, Freund GG, et al. From inflammation to sickness and depression: when the immune system subjugates the brain. Nat Rev Neurosci. 2008;9:46–56.View ArticlePubMedPubMed CentralGoogle Scholar
- Ponomarev ED, Veremeyko T, Barteneva N, et al. MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the C/EBP-[alpha]-PU. 1 pathway. Nat. Med. 2011;17:64–70.Google Scholar
- Block ML, Zecca L, Hong JS. Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nat Rev Neurosci. 2007;8:57–69.View ArticlePubMedGoogle Scholar
- Erblich B, Zhu L, Etgen AM, et al. Absence of colony stimulation factor-1 receptor results in loss of microglia, disrupted brain development and olfactory deficits. PLoS One. 2011;6:e26317.View ArticlePubMedPubMed CentralGoogle Scholar
- Elmore MRP, Najafi AR, et al. Colony-stimulating factor 1 receptor signaling is necessary for microglia viability, unmasking a microglia progenitor cell in the adult brain. Neuron. 2014;82:380–97.View ArticlePubMedPubMed CentralGoogle Scholar
- Olmos-Alonso A, Schetters STT, Sri S, et al. Pharmacological targeting of CSF1R inhibits microglial proliferation and prevents the progression of Alzheimer's-like pathology. Brain. 2016;139:891–907.View ArticlePubMedPubMed CentralGoogle Scholar
- Ries CH, Cannarile MA, Hoves S, et al. Targeting tumor-associated macrophages with anti-CSF-1R antibody reveals a strategy for cancer therapy. Cancer Cell. 2014;25:846–59.View ArticlePubMedGoogle Scholar
- Rupprecht R, Papadopoulos V, Rammes G, et al. Translocator protein. (18 kDa)(TSPO) as a therapeutic target for neurological and psychiatric disorders. Nat. Rev. Drug Discov. 2010;9:971–88.View ArticlePubMedGoogle Scholar
- Hannestad J, Gallezot JD, Schafbauer T, et al. Endotoxin-induced systemic inflammation activates microglia: [11C]PBR28 positron emission tomography in nonhuman primates. NeuroImage. 2012;63:232–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Sandiego CM, Gallezot JD, Pittman B, et al. Imaging robust microglial activation after lipopolysaccharide administration in humans with PET. Proc Nat Acad Sci USA. 2015;112:12468–73.View ArticlePubMedPubMed CentralGoogle Scholar
- Artis D, Bremer R, Gillette S, et al. Molecular scaffolds for kinase ligand development. USA: Patent US-20050164300; 2005.Google Scholar
- Innis RB, Cunningham VJ, Delforge J, et al. Consensus nomenclature for in vivo imaging of reversibly binding radioligands. J Cereb Blood Flow Metab. 2007;27:1533–9.View ArticlePubMedGoogle Scholar
- Ichise M, Liow JS, Lu JQ, et al. Linearized reference tissue parametric imaging methods: application to [11C]DASB positron emission tomography studies of the serotonin transporter in human brain. J Cereb Blood Flow Metab. 2003;23:1096–112.View ArticlePubMedGoogle Scholar
- Imaizumi M, Briard E, Zoghbi SS, et al. Brain and whole-body imaging in nonhuman primates of [11C]PBR28, a promising PET radioligand for peripheral benzodiazepine receptors. NeuroImage. 2008;39:1289–98.View ArticlePubMedGoogle Scholar
- Park E, Gallezot J-D, Delgadillo A, et al. 11C-PBR28 imaging in multiple sclerosis patients and healthy controls: test-retest reproducibility and focal visualization of active white matter areas. Eur J Nucl Med Mol Imaging. 2015;42:1081–92.View ArticlePubMedGoogle Scholar