- Original research
- Open Access
In vivo characterization of the novel CD44v6-targeting Fab fragment AbD15179 for molecular imaging of squamous cell carcinoma: a dual-isotope study
EJNMMI Research volume 4, Article number: 11 (2014)
Patients with squamous cell carcinoma in the head and neck region (HNSCC) offer a diagnostic challenge due to difficulties to detect small tumours and metastases. Imaging methods available are not sufficient, and radio-immunodiagnostics could increase specificity and sensitivity of diagnostics. The objective of this study was to evaluate, for the first time, the in vivo properties of the radiolabelled CD44v6-targeting fragment AbD15179 and to assess its utility as a targeting agent for radio-immunodiagnostics of CD44v6-expressing tumours.
The fully human CD44v6-targeting Fab fragment AbD15179 was labelled with 111In or 125I, as models for radionuclides suitable for imaging with SPECT or PET. Species specificity, antigen specificity and internalization properties were first assessed in vitro. In vivo specificity and biodistribution were then evaluated in tumour-bearing mice using a dual-tumour and dual-isotope setup.
Both species-specific and antigen-specific binding of the conjugates were demonstrated in vitro, with no detectable internalization. The in vivo studies demonstrated specific tumour binding and favourable tumour targeting properties for both conjugates, albeit with higher tumour uptake, slower tumour dissociation, higher tumour-to-blood ratio and higher CD44v6 sensitivity for the 111In-labelled fragment. In contrast, the 125I-Fab demonstrated more favourable tumour-to-organ ratios for liver, spleen and kidneys.
We conclude that AbD15179 efficiently targets CD44v6-expressing squamous cell carcinoma xenografts, and particularly, the 111In-Fab displayed high and specific tumour uptake. CD44v6 emerges as a suitable target for radio-immunodiagnostics, and a fully human antibody fragment such as AbD15179 can enable further clinical imaging studies.
Squamous cell carcinoma of the head and neck region (HNSCC) makes up approximately 5% of all solid cancers in Europe and North America. In 2008, the worldwide incidence of oral cavity cancer was estimated to 263,900 cases . Squamous cell carcinoma (SCC) is the most common histopathological type in head and neck cancer and occurs in the oral cavity, pharynx, sino-nasal cavity and larynx. The oral cavity is presently the predominant site, although pharyngeal cancer is becoming more common due to infections with human papilloma viruses [2, 3]. Still, tobacco use is the outstanding risk factor and particularly in combination with alcohol [4, 5].
Already at diagnosis, 60% of HNSCC patients have metastatic disease , most commonly locoregional metastasis in the lymph nodes in the neck. Treatment of HNSCC generally involves several modalities in combination such as surgery and radiation and, in some cases, chemotherapy and targeted therapy using antibodies. Despite the fact that treatments have improved, there is still a high risk of disease recurrence. In the last 30 years, survival rates have only improved slightly, with the exception of HPV-induced carcinomas .
A cornerstone in the work-up of patients with HNSCC is accurate imaging of the primary tumour and metastases, since tumour stage strongly correlates to prognosis and hence the chosen treatment. The imaging methods available in clinical use today are computed tomography (CT), magnetic resonance imaging (MRI), ultrasound and fludeoxyglucose-positron emission tomography/CT (FDG-PET/CT). FDG-PET/CT is a valuable method when searching for metastases and primary tumours. However, its specificity is limited due to increased FDG uptake in benign lymphadenitis, other inflammatory processes or after treatment. Also, small clusters of cancer cells can escape diagnosis due to the limiting size factor of the PET/CT technique . More specific imaging techniques for HNSCC could both improve the delineation of the primary tumour for more precise surgical removal and more accurately find lymph node metastases.
One promising method that may improve the management of patients with head and neck malignancies is radio-immunodiagnostics, which combines the high sensitivity and resolution of a PET or single-photon emission tomography (SPECT) camera with the tumour specificity of an antibody or antibody fragment. Such radiolabelled monoclonal antibodies (mAbs) can be utilized not only in the detection of metastatic lesions and primary tumours but also in applications that include monitoring of tumour response to therapy, dosimetric calculations, and therapy . Although intact mAbs are still most commonly used, they are not considered optimal for radio-immunodiagnostics. Their relatively large size (approximately 150 kDa) tends to cause unfavourable imaging kinetics due to slow plasma clearance, poor tumour penetration and higher risk of eliciting a host antibody response [10–12]. One solution to these obstacles has been to reduce the size of intact antibodies to smaller fragments, achieved either through enzymatic cleavage or by genetic engineering. For instance, a Fab fragment is an antibody structure of about 50 kDa that still binds to antigens but is monovalent with no Fc portion, whereas Fab2 fragment antibodies have two antigen-binding Fab portions linked together by disulphide bonds and therefore are divalent with a molecular weight of about 110 kDa. Fragments of the intact antibody may exhibit shorter half-life, faster blood clearance and better tumour penetration, resulting in faster and higher signal-to-noise ratio. Furthermore, the removal of the Fc segment could reduce the non-specific distribution in vivo of the mAb via Fc receptors found on normal cells . However, reduction in size can also reduce antibody avidity , and the shortened serum half-life, likely due to kidney clearance and lack of Fc-mediated neonatal receptor recycling, may decrease the overall tumour uptake of these small molecules .
Receptors on the surface of cells can serve as targets for antibodies and antibody fragments, and if they are expressed specifically by tumour cells, they are excellent targets for radio-immunodiagnostics. There are several promising receptors for radio-immunodiagnostics such as EGFR and isoforms of CD44. CD44 belongs to a family of glycoproteins serving as surface receptors for extracellular matrix components, mainly hyaluronic acid. The receptors are involved in migration and adhesion of cells. Twenty exons encode CD44, and exons 6 to 15, namely variable exons 1 to 10 (v1 to v10), can be alternatively spliced with diverse end products . Most tissues, both epithelial and non-epithelial, express variants of CD44 with the exception of splice variants v4, v6 and v9 which are more sparsely occurring . For CD44v6, the expression in normal tissue is restricted to squamous and transitional epithelium [17, 18]. The overexpression of certain CD44 splice variants has been found to be involved in cancer progression, and CD44v6 in particular has been suggested to play a role in tumour formation, invasion, and metastasis formation [16, 19]. One proposed mechanism for the increased metastatic potential is binding to extracellular matrix components, enabling invasion and angiogenesis [19, 20]. Previous studies have shown overexpression of CD44v6 in squamous cell carcinomas, for example, in the head and neck, lung, skin, oesophagus, cervix and papillary thyroid cancers, and several studies have demonstrated overexpression of CD44v6 in over 90% of primary and metastatic HNSCC [19, 21]. This makes CD44v6 a promising candidate marker for targeting of squamous cell carcinoma . A chimeric monoclonal antibody, cMAb U36, targeted at CD44v6 has previously been evaluated both for diagnostic and therapeutic uses with promising results [23–25], as well as with a fully humanized version, BIWA-4, binding to an overlapping epitope in the v6 domain [26, 27]. In a previous study, chimeric Fab and Fab2 fragments of U36 radiolabelled with 125I were characterized in vitro and in vivo and compared to the intact antibody. Tumour-to-blood ratios and tumour penetration were increased for Fab and Fab2 compared with the intact antibody .
To date, few antibody fragments toward CD44v6 have been reported, and none of them are fully human with a thoroughly characterized binding site. Thus, to facilitate improved targeting of CD44v6, we have selected in vitro characterized fully human Fab fragments, derived from the HuCAL PLATINUM library, which specifically recognize v6-containing isoforms of CD44 . Clones derived from such recombinant antibody repertoires provide a renewable source of human antibodies or antibody fragments that can be expressed in Escherichia coli. Furthermore, the recombinant origin of the antibody fragments enables future reformatting to provide molecules that are optimized for either diagnostic or therapeutic applications . One main candidate, AbD15179, demonstrated optimal affinity and slow dissociation from the tumour cells and was chosen for further evaluation. To our knowledge, this study generated the first and most thoroughly characterized fully human Fab fragment specific for CD44v6 .
The aim of this study was primarily to assess the in vivo tumour targeting capabilities of the novel, fully human, CD44v6-targeting antibody fragment AbD15179. The Fab fragment was first evaluated for species specificity using surface plasmon resonance (SPR) and was then labelled with 111In or 125I, as models for radionuclides suitable for imaging with SPECT or PET. Specific binding and internalization of labelled conjugates was evaluated in CD44v6-expressing SCC cells in vitro, and in vivo binding specificity and biodistribution studies were then performed using 111In- or 125I-labelled Fab fragments in a dual-isotope study in tumour-bearing mice with xenografts of varying CD44v6 expression.
Antibody fragment AbD15179
The CD44v6-binding Fab fragment AbD15179 was supplied from AbD Serotec (Kidlington, UK). It was selected from an array of 13 different human antibody fragments, all recognizing CD44v6. The selection and production of this antibody fragment have been described previously . The native Fab fragment is referred to as AbD15179 throughout this paper. AbD15179 was supplied in 3× PBS (0.72 μg/ml) and stored at −80°C. The fragment used for 111In-labelling was separated by size-exclusion chromatography on a NAP-5 column (Amersham Biosciences, Uppsala, Sweden) pre-equilibrated with purified (MilliQ, Millipore Corp., Billerica, MA, USA) water. It was then freeze-dried and stored at −20°C before use in order to facilitate buffer exchange and concentration adjustments.
Biosensor evaluation of species specificity of AbD15179
Two 16-mer peptides corresponding to the human (CGNRWHEGYRQTPREDS) and mouse (CQNGWQGKNPPTPSEDS) forms of the CD44v6 antigen used to generate AbD15179 were produced by peptide synthesis (Elim Biopharmaceuticals, Hayward, CA, USA). The peptide antigens were individually conjugated to bovine serum albumin (BSA) through N-terminal cysteine residues. Purified recombinant CD44v3-10 (see  for details) and the two carrier-conjugated peptides were immobilized on a ProteOn XPR36 general layer medium biosensor chip (Bio-Rad, Hercules, CA, USA) using amine coupling. This yielded immobilization levels of approximately 1,000 (CD44 isoform) and 300 (BSA-peptides) response units, respectively. A CD44 isoform that lacks the v6 region (CD44v3-10Δv6) was included as a negative control in one channel. Dilution series of AbD15179 (0.6 to 50 nM) were injected at 25°C with a flow rate of 50 μl/min. PBS containing 0.05% Tween 20 (pH 7.4) was used as running buffer, and the surfaces were regenerated using 10 mM HCl. The chimeric anti-CD44v6 monoclonal antibody U36  was used as a positive control. Binding curves were normalized using a blank ligand channel and a parallel buffer injection prior to fitting to a 1:1 Langmuir isotherm using the ProteOn manager software version 18.104.22.168 (Bio-Rad).
The human HNSCC cell line H314 (obtained from European Collection of Cell Cultures, Salisbury, UK), derived from floor of mouth, was cultured in a one-to-one mixture of Ham's F12 and Dulbecco's modified eagle medium (DMEM), supplemented with 10% foetal calf serum, 2 mM l-glutamine, and antibiotics (100 IU penicillin and 100 μg/ml streptomycin). H314 has demonstrated an average antigen density of approximately 650,000 CD44v6 receptors . The SCC cell line A431 (obtained from the American Type Culture Collection, Manassas, VA, USA), derived from vulva, was cultured in Ham's F10, with the same supplements. A431 has been shown to be a highly CD44v6-expressing cell line with approximately 3,000,000 receptors per cell . As a negative control, the breast cancer cell line MDA-MB-231 (obtained from the American Type Culture Collection) was used. It was cultured in DMEM, with the same supplements as above. This cell line has demonstrated no detectable CD44v6 expression . The cells were incubated at 37°C in an atmosphere containing humidified air with 5% CO2. The cells used in the monolayer culture experiments were trypsinized and seeded in separate dishes used for experiments 2 to 3 days prior to the studies.
125I and 111In labelling of AbD15179
125I- and 111In-labelled AbD15179 have previously been extensively evaluated in vitro. In the present study, AbD15179 was labelled with 125I (PerkinElmer, Waltham, MA, USA) using direct chloramine T labelling  (Sigma-Aldrich, St. Louis, MO, USA) as previously described [28, 31]. Generally, a stock solution of 10 MBq 125I was mixed with 100 μl PBS, and 100 μg of the Fab fragment (140 μl, 0.72 mg/ml in PBS) was added. The reaction was initiated by the addition of chloramine T (Sigma-Aldrich) in water (40 μl, 4 mg/ml) and was quenched after a 5-min incubation on ice by the addition of sodium metabisulphite (Merck, Whitehouse Station, NJ, USA) in water (80 μl, 4 mg/ml). Each labelled protein was separated from non-reacted 125I and low-molecular-weight reaction components on a NAP-5 column pre-equilibrated with PBS. 125I-labelled AbD15179 is referred to as 125I-Fab in this paper.
Labelling of AbD15179 with 111In (Mallinckrodt Medical B.V., St. Louis, MO, USA) was done using CHX-A″-DTPA labelling as described previously [31, 34]. The chelator CHX-A″-DTPA used for 111In labelling exhibits high affinity for several isotopes that are suitable for either diagnostic or therapeutic purposes, including 111In, 86Y, 90Y and 177Lu. In short, to 75 μg AbD15179 solution (15 μl, 5 mg/ml in borate buffer, pH 9), 4.1 μl of CHX-A″-DTPA solution (1 mg/ml in borate buffer, 0.07 M, pH 9) was added, corresponding to a chelator-to-AbD15179 molar ratio of 5:1. The reaction mixture was incubated overnight at room temperature, and the conjugated antibody was then separated from free CHX-A″-DTPA using a NAP-5 column equilibrated with acetate buffer (0.2 M, pH 5.5). Approximately 10 MBq of 111In in acetate buffer was then added to the conjugated AbD15179, and the reaction mixture was allowed to incubate for 30 min at room temperature. Labelled Fab fragment was then separated from the non-reacted radionuclide and low-molecular-weight reaction components by using a NAP-5 column pre-equilibrated with PBS. 111In-labelled AbD15179 is referred to as 111In-Fab in this paper.
To determine the purity and stability of the labelled antibody fragments, instant thin-layer chromatography (ITLC) was performed on 125I- and 111In-labelled conjugates. The purity of the samples taken immediately, as well as stored at 4°C for 48 h, was analysed. Approximately 1 μl of the conjugate was placed on a chromatography strip (Biodex, Shirley, NY, USA) and placed into a ‘running buffer’ (70% acetone or 0.2 M citric acid for 125I and 111In, respectively), followed by measurements on a Cyclone Storage Phosphor System (PerkinElmer). The data were analysed using the OptiQuant image analysis software (PerkinElmer). Furthermore, stability in murine serum was evaluated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis (200 V constant) on NuPAGE® 4-12% Bis-Tris gel (Novex, Thermo Fisher Scientific, Waltham, MA, USA). Briefly, 1.8 μg of 125I-Fab or 111In-Fab was incubated in 120 μl of murine serum (42% in PBS, pH 7.4) for 60 min at 37°C. After incubation, the samples were treated with NuPAGE® LDS Sample Buffer according to the manufacturer's instructions and loaded on the gel. One well was loaded with 4 μl of HiMark™ Pre-Stained Protein Standard (Novex®). Measurements were performed on a Cyclone Storage Phosphor System, and the data were analysed using the OptiQuant image analysis software.
In vitro binding, specificity and internalization
In vitro binding and specificity were assessed for 125I-Fab, and binding, specificity and internalization were assessed for 111In-Fab using both H314 and A431 cells. For binding and specificity measurements, 10 nM 125I-Fab or 111In-Fab was added to each dish and incubated for 2 to 24 h. To some of the dishes, a 100-time molar excess of unlabelled antibody fragment was simultaneously added for assessment of binding specificity. After incubation, the cells were washed with serum-free medium to remove unbound antibody fragment, detached with 0.5 ml trypsin at 37°C and resuspended in 1 ml complete medium. A sample of 0.5 ml of the cell solution was taken for cell counting, and the remaining 1 ml was collected for radioactivity measurements.
For internalization measurements, 10 nM 111In-Fab was added to each dish and incubated for 2 to 24 h using the following conditions: (i) incubation at 37°C to allow internalization of the conjugate, (ii) incubation at 37°C in the presence of a 100-time molar excess of unlabelled antibody for assessment of binding specificity and (iii) incubation at 0°C for correction of acid-resistant surface binding at non-internalizing conditions. After incubation, the cells were washed with serum-free medium to remove unbound antibody and incubated at 0°C with 20 mM HCl/150 mM NaCl (pH 1.7) for 15 min to release antibodies bound to the cell surface. The supernatant was collected, and the radioactivity was counted in a gamma counter to assess the amount of membrane-bound conjugates. Finally, the cells were lysed with 0.1 M NaOH/1% Triton X-100, and the radioactivity was measured in a gamma counter to assess the amount of internalized conjugates. After correction for acid-resistant surface binding, the internalization at 37°C was calculated as the percentage of the total amount of radioactivity specifically bound (internalized and surface-bound) to the cells.
In vivo specificity
Tumour xenografts were formed by subcutaneous inoculation of approximately 8 × 106 A431 cells (high CD44v6 expression) suspended in 300 μl injected medium in the right posterior leg, and approximately 8 × 106 MDA-MB-231 cells (no CD44v6 expression) in the left posterior leg, in five nude balb/c (nu/nu) female mice. The mice were housed in a controlled environment and fed ad libitum. All experiments complied with the current Swedish law and were performed with permission granted by the Uppsala Committee of Animal Research Ethics.
After allowing the tumours to grow for 2 weeks, the animals were injected with125I-Fab (5 μg in 200 μl PBS, 100 kBq, rendering a specific activity of 20 kBq/μg) in the tail vein. After 24 h, the animals were euthanized with a mixture of ketamine and xylazine followed by heart puncture, and blood was aspirated and weighed. Tumours were excised and weighed. Radioactivity content of the blood and tumours was measured in a gamma counter, together with three injection standards of 200 μl 125I-Fab (volume of injection). A mean injected dose was calculated from the injection standards, and the injected activity of 125I-Fab for each mouse was calculated by subtracting the residual activity in the syringe from the mean injected dose. Radioactivity uptake in the organ was calculated as percent of injected activity per gram of tissue (%ID/g). Tumour-to-organ ratio was calculated as activity/gtumour divided by activity/gorgan.
Tumour xenografts of A431 and H314 cells (high and moderate CD44v6 expression, respectively) were formed in 20 nude balb/c (nu/nu) female mice as described above. The mice were housed in a controlled environment and fed ad libitum. The experiments complied with the current Swedish law and were performed with permission granted by the Uppsala Committee of Animal Research Ethics.
Three weeks after tumour cell injection, the animals received an intravenous injection via the tail vein with125I-Fab (100 kBq, specific activity of 20 kBq/μg) and 111In-Fab (100 kBq, specific activity of 20 kBq/μg), totally 5 μg Fab fragments in 200 μl PBS. At 6 h (N = 5), 24 h (N = 5), 48 h (N = 5) and 72 h (N = 5) post injection, the animals were euthanized with a mixture of ketamine and xylazine followed by heart puncture, and blood was aspirated and weighed. The tumours, salivary glands, thyroid (en bloc with larynx), tongue, liver, kidneys, spleen, urinary bladder, colon, upper gastrointestinal tract, skin, bone and muscle were excised and weighed, and radioactivity was measured in a gamma counter. The tail and the rest of the body were also separated, weighed and measured in a gamma counter, together with three injection standards for each group. The injected dose was calculated as described above. Radioactivity uptake in the organ was calculated as %ID/g. Uptake was calculated as percent of injected activity per organ. Tumour-to-organ ratio was calculated as activity/gtumour divided by activity/gorgan.
Statistical analyses were performed using GraphPad Prism Version 5.02 for Windows (GraphPad Software, Tumour Biol Inc., La Jolla, CA, USA, http://www.graphpad.com). For in vivo specificity studies, the differences in 125I-Fab uptake between MDA-MB-231 tumours and A431 tumours were assessed using a two-tailed paired t test and were considered statistically significant if P < 0.05. In the biodistribution studies, the data are presented as the mean ± standard deviation (SD). The significance of differences between the groups over time was tested with one-way analysis of variance (ANOVA) with Newman-Keuls multiple comparison test. The differences were considered statistically significant if P < 0.05. The differences in 125I-Fab or 111In-Fab uptake between H314 tumours and A431 tumours were assessed using repeated measures ANOVA with Newman-Keuls multiple comparison test and were considered statistically significant if P < 0.05.
Biosensor evaluation of species specificity of AbD15179
The Fab fragment AbD15179 bound strongly to the human v6 peptide, whereas no binding was detected for the murine form of the target (Figure 1). The same binding pattern was observed for U36 as expected. The low nanomolar target binding affinity (KD < 7 nM) of AbD15179 reported previously  was verified using triplicate injections and two different immobilization levels of CD44v6-10. As observed earlier, the affinity for the conjugated peptide target was roughly tenfold lower than that for the full-length extracellular domain CD44v3-10, which was a result of both a faster dissociation rate constant and a slower association.
Labelling yields for 125I and 111In labelling of AbD15179 for in vitro studies were 50% and 39%, rendering specific activities of 50 and 52 kBq/μg, respectively. The labelling yield for 125I labelling of AbD15179 for in vivo specificity studies was 66%, and for biodistribution studies 74%, rendering specific activities of 66 and 74 kBq/μg, respectively. Specific activities were adjusted to 20 kBq/μg for all in vivo conjugates, using unlabelled AbD15179, before injections. The purity of the labelled proteins was >99.9% according to the ITLC analysis. The labelling yield for 111In labelling of AbD15179 for biodistribution studies was 63%, resulting in a specific activity of 84 kBq/μg, and the purity of the labelled protein was >96% according to the ITLC analysis. The purity of labelled conjugates stored in PBS at 4°C was re-analysed 48 h post injection (p.i.) and was unchanged according to the ITLC analysis. SDS-PAGE analysis of the sample incubated in serum for 1 h at 37°C showed a single radioactivity peak corresponding to the size of the intact Fab fragment for both 125I-Fab and 111In-Fab, indicating high stability of the fragments.
In vitro binding, specificity and internalization
Cellular binding of 111In-Fab and 125In-Fab increased with time, levelling out at approximately 6 h in both cell lines. Furthermore, binding of 111In-Fab and 125In-Fab was blocked by an excess of unlabelled AbD15179 in both cell lines, demonstrating specific binding of the conjugates (Figure 2). Internalization studies of 111In-Fab demonstrated no detectable internalization after 24 h in neither A431 nor H314 cells. Furthermore, 125I- and 111In-labelled AbD15179 have previously been extensively evaluated in vitro. In brief, both 125I-Fab and 111In-Fab bound specifically to CD44v6-expressing cells and demonstrated a general biphasic appearance of one high- and one low-affinity binding event. The population of high-affinity binders was significantly higher for 111In-Fab than that for 125I-Fab, which resulted in longer retention of 111In-Fab compared to 125I-Fab on SCC cells. The equilibrium dissociation constant (KD) for the high-affinity population (measured using cell-based real-time measurements and interaction analysis) was approximately 8 ± 2 nM and did not significantly differ between 125I- or 111In-labelled AbD15179 on the H314 or A431 cells .
In vivo specificity
The specificity of AbD15179 was assessed in vivo by comparing the uptake of 125I-Fab in CD44v6-negative (MDA-MB-231) and CD44v6-positive (A431) tumours 24 h p.i. in tumour-bearing mice. At the time of the experiments, median tumour weight for MDA-MB-231 tumours was 131 mg, generally ranging between 90 mg (lower quartile) and 338 mg (upper quartile). For the A431 tumours, the median weight was 62 mg, generally ranging between 43 and 95 mg. There was no correlation between the tumour weight and %ID/g of 125I-Fab. Tumour uptake differed significantly between the CD44v6-negative and CD44v6-positive tumours, with the CD44v6-positive A431 tumours displaying more than two times higher uptake than the CD44v6-negative MDA-MB-231 tumours (0.66 ± 0.13 (%ID/g ± SD) and 1.34 ± 0.26 %ID/g for MDA-MB-231 and A431 tumours, respectively).
The biodistribution of AbD15179 was assessed in vivo by comparing the uptake of 125I-Fab and 111In-Fab in mice bearing both CD44v6-moderate (H314) and CD44v6-positive (A431) tumours. At the time of the experiments, the median tumour weight for the H314 tumours was 23 mg, generally ranging between 17 mg (lower quartile) and 33 mg (upper quartile), and the median tumour weight for the A431 tumours was 105 mg, generally ranging between 86 and 294 mg. There was no correlation between tumour weight and %ID/g of 125I-Fab or 111In-Fab. The biodistribution data of the selected organs of the 125I-Fab and 111In-Fab fragments in tumour-bearing mice are shown in Figure 3. The selected organs were the ones in the vicinity of the head and neck region, tongue, salivary glands and thyroid, as well as the organs of high interest for radio-immunotargeting such as liver, kidneys and spleen. Generally, 111In-Fab displayed not only higher uptake of radioactivity and slower clearance in most organs compared with 125I-Fab but also a higher tumour-to-blood ratio and a clearer discrimination between high- and moderate-CD44v6-expressing tumours. The biodistribution data are described in more detail below.
For 111In-Fab, the activity in tumours remained relatively stable over time in both the moderate (H314) and high (A431) CD44v6-expressing tumour models, with no significant changes in tumour uptake over time (Figure 3A). 111In-Fab displayed a clear difference in tumour uptake between the moderate- and high-CD44v6-expressing tumours, ranging between 1.5 to 2 times higher for the high-CD44v6-expressing A431 tumours (calculated for each individual mouse) throughout the study. At 6 h p.i., the percentage of the injected dose per gram of 111In-Fab in the moderate-CD44v6-expressing H314 tumours was 2.4 ± 1.1 (SD) and remained at this level. The uptake in the high-CD44v6-expressing A431 tumours was significantly higher, being 4.6 ± 1.9 %ID/g at 6 h p.i., with a slight, although not significant, decrease over time to 3.2 ± 0.2 %ID/g at 72 h p.i. For blood, bone and organs, 111In-Fab demonstrated highest activities at the first time point (6 h), declining with time. The kidneys displayed the highest activity (131 ± 47 %ID/g at 6 h), which rapidly decreased to 67 ± 11 %ID/g by 24 h. The second highest normal organ uptake was observed in the liver (13 ± 5.6 %ID/g at 6 h), rapidly decreasing to 5.6 ± 1.4 %ID/g by 24 h.
125I-Fab generally displayed lower uptake values than 111In-Fab in both tumours and normal organs, with a more pronounced clearance and decrease in activity over time (Figure 3B). At 6, 24, and 48 h p.i., 125I-Fab displayed a difference in tumour uptake between the high- and moderate-CD44v6-expressing tumours, although not as pronounced as for 111In-Fab, ranging between 1.3 and 1.4 times higher for the high-CD44v6-expressing A431 tumours compared to the medium-CD44v6-expressing H314 tumours. At 72 h p.i., there was no difference in activity between the tumours. A clear decrease in tumour activity was seen from 6 to 24 h p.i. in both tumour models. In high-CD44v6-expressing A431 tumours, this decrease was also significant between 48 and 72 h p.i. At 6 h p.i., the activity of 125I-Fab in the moderate-CD44v6-expressing tumours was 1.1 ± 0.6 %ID/g, steadily decreasing over time, reaching 0.25 ± 0.07 %ID/g at 72 h p.i. In the high-CD44v6-expressing A431 tumours, the activity of 125I-Fab was 1.6 ± 0.1 %ID/g at 6 h, decreasing to 0.22 ± 0.03 %ID/g at 72 h p.i. As for the 111In conjugate, the organ with the highest activity was the kidney (6.5 ± 2.1 %ID/g at 6 h), with the activity rapidly decreasing to 1.4 ± 0.2 at 24 h. The stomach and salivary glands displayed high initial values, both rapidly decreasing to below 0.3 %ID/g 48 h p.i. The exception to this trend was uptake in the thyroid, where no decrease in activity was observed (Figure 3B, inset).
In order to assess the selectivity of tumour targeting for the 111In-Fab and 125I-Fab conjugates, tumour-to-normal tissue ratios were calculated based on the high-CD44v6-expressing A431 tumours (Figure 4), and tumour-to-blood ratios were calculated for both tumour types (Figure 4, insets). The tumour-to-organ ratios for 111In-Fab increased over time for most organs of interest including the blood (Figure 4A) and were highest at 48 and 72 h p.i. The tumour-to-blood ratios for both A431 and H314 tumours increased over time with a ratio of over 10 for A431 tumours at 72 h p.i. (Figure 3A, inset). Also, for 125I-Fab, the tumour-to-organ ratios increased over time (Figure 3B). The tumour-to-blood ratios increased with time for both tumours with a maximum ratio at 72 h above 4 in both A431 and H314 tumours (Figure 3B, inset). In the spleen, liver and kidney, higher tumour-to-normal tissue ratios generally were observed with 125I-Fab, while the opposite behaviour was seen in the blood, tongue and salivary glands, where 111In-Fab displayed higher ratios.
Detecting small metastases and small primary tumours in patients with HNSCC could improve staging and consequently also the treatment of these patients. Tumour stage correlates closely to survival, and if treatment can be individualized through better staging, survival rates should improve. Although there are several diagnostic methods available today, major difficulties in finding small tumours and detecting minimal residual disease still remain. A specific targeting molecule labelled with an appropriate radionuclide utilized in a SPECT or PET setting could add considerably to existing methods. This kind of molecular imaging offers the ability to visualize, characterize and measure processes on molecular and cellular levels non-invasively in living systems. Additionally, fused imaging modalities such as PET/CT, PET/MRI and SPECT/CT have enabled the combination of anatomy and molecular events into a single image . The antibody fragment AbD15179 recognizes the CD44v6 antigen, which has shown consistent expression in many primary squamous cell carcinomas but only in a subset of normal tissues . The potential of CD44v6 as a target for radio-immunotargeting agent has been demonstrated in previous studies using full-length antibodies [23, 24]. However, very few antibody fragments toward this target have been reported, and none of them are fully human with a thoroughly characterized binding site. This study represents, to our knowledge, the first in vivo evaluation of a fully human CD44v6-targeting antibody fragment.
The objective of this study was to evaluate, for the first time, the in vivo properties of the CD44v6-targeting fragment AbD15179, labelled with 111In or 125I, and to assess its utility as a targeting agent for radio-immunodiagnostics of CD44v6-expressing tumours. Species specificity, antigen specificity and internalization properties were first assessed in vitro. In vivo specificity and biodistribution was then evaluated in tumour-bearing mice, using a dual-tumour and dual-isotope setup. An advantage of using this setup is the possibility to evaluate uptake in a low/moderate-CD44v6-expressing tumour vs. a high-CD44v6-expressing tumour, normalizing each dataset to the same mouse. This can minimize the effects of potential differences among groups of experimental animals such as tumour size and injection variability. In the same way, the effects of 111In vs. 125I labelling of the fragment can be evaluated.
In vitro, both species-specific and antigen-specific binding of the conjugates were demonstrated, with no detectable internalization. The lack of binding of AbD15179 to the murine v6 peptide was expected, since 9 of the 16 residues in the peptides differ between the murine and human variants . Furthermore, the two consecutive proline residues, which are not present in the human sequence, may distort the conformation of murine CD44v6 in the targeted region. The low internalization was also in line with previous studies of CD44v6, which have shown that CD44v6 is commonly not an internalizing receptor [32, 36]. Generally, high tumour internalization of a radioconjugate is considered beneficial for radiometals such as 111In, since internalization of a residualizing radiometal will cause long tumour retention times, often outweighing disadvantages of radiometals such as accumulation in the liver, spleen and kidneys . Thus, the lack of internalization shown in vitro could favour a radiohalogen before a radiometal as a radiolabel in vivo for AbD15179. However, in a previous in vitro study of this fragment, we could demonstrate that the binding characteristics of 111In-Fab were more favourable than those for 125I-Fab, even in a non-internalizing setting, resulting in longer retention of 111In-Fab compared to that of 125I-Fab on SCC cells . Thus, we considered 111In-Fab to be an interesting candidate for the subsequent in vivo studies, despite an expected low internalization rate.
In vivo, we first demonstrated a CD44v6-dependent tumour uptake for AbD15179, with a markedly higher tumour uptake of 125I-Fab in high-CD44v6-expressing tumours compared to the CD44v6-negative tumours, establishing the specificity of the conjugate also in vivo. Tumour uptake (%ID/g) differed significantly between the CD44v6-negative and CD44v6-positive tumours, with the CD44v6-positive A431 tumours displaying more than two times higher uptake than the CD44v6-negative MDA-MB-231 tumours. This is in line with the values obtained at the same time point (24 h p.i.) in the subsequent biodistribution study in this paper, in which high-CD44v6-expressing A431 tumour uptake of 125I-Fab was approximately 1.3 times higher than the uptake in the moderate-CD44v6-expressing H314 tumours. However, at later time points, the difference in tumour uptake between moderate- and high-CD44v6-expressing tumours was diminished for 125I-Fab, whereas 111In-Fab displayed a clear difference in tumour uptake between the moderate- and high-CD44v6-expressing tumours, ranging between 1.5 to 2 times higher for the high-CD44v6-expressing tumours throughout the study. Thus, we conclude that 111In-Fab displayed higher in vivo target sensitivity at the administered dose.
111In-Fab also demonstrated a higher tumour uptake than 125I-Fab at all time points, being more than 15 times higher at the last time point (72 h p.i.). The accumulation and slower dissociation of 111In-Fab in tumours were in line with the slower off-rate of 111In-Fab compared to 125I-Fab observed on these tumour cells in vitro shown in a preceding study  using non-internalizing conditions, as discussed previously. However, the effect seen in vivo could nevertheless in part be due to some internalization of the 111In-Fab by the tumour cells as the cellular membrane renews itself, even though our in vitro experiments showed no detectable internalization of 111In-Fab into either H314 or A431 tumour cells up to 24 h of incubation at 37°C.
For successful imaging, the contrast between tumour and surrounding tissue is of even higher importance than the total tumour uptake. In the present study, tumour-to-blood ratios were above 1 already at 24 h p.i. for both conjugates and continued to increase throughout the study. This can be compared to previous studies, where successful CD44v6 imaging in tumour-bearing mice using both 111In  and 124I  coupled to a CD44v6-targeting antibody was performed, despite three to four times lower tumour-to-blood ratios at the time of imaging than in our current study. We believe that this is very promising and demonstrates that imaging with 111In- or 124I-labelled AbD15179 is indeed feasible.
In general, 125I-labelled Fab fragments were cleared from the circulation more rapidly than the 111In-Fab fragments. This is in line with several other studies using 125I- and 111In-labelled molecules, in which the 125I-labelled compounds have generally shown lower uptake and faster clearance from organs. The reason for this has been suggested to be dehalogenation of the radioiodinated protein or exchange of 111In into iron-binding proteins . Another explanation for the more rapid clearance could be a higher amount of aggregation of 125I-Fab than for 111In-Fab , resulting in an increase in the effective size and, thus, clearance from the circulation via a size-dependent mechanism, as has been shown previously for liposomes .
Furthermore, 111In-Fab displayed a considerably higher uptake than 125I-Fab in the liver, spleen and kidneys. These observations are also in agreement with those reported in the literature when 125I and 111In are compared . The observed increase of 111In in the liver could be a result of antibody clearance or exchange with iron-binding protein or a mixture of both processes. The accumulation of 111In in the kidneys can probably be attributed to the active filtration of the fragments and probably to the subsequent exchange of 111In into non-binding proteins within this organ . The property of the liver and kidneys to accumulate 111In and clear it more slowly is a distinct disadvantage of 111In and may limit its use, although this should be balanced by the fact that for 111In-Fab, the magnitude of tumour uptake was several times higher than seen with the radioiodinated fragment. Additionally, several preclinical and clinical experiments have shown that undesirable renal uptake of 111In can be reduced with pretreatment with e.g. cationic amino acids such as lysine [42–44]. Moreover, with the aim to discriminate between tumour/metastases and surrounding tissue in the head and neck cancer region, renal uptake is of less concern for imaging of head and neck cancer, although it may limit the amount of activity that can be administered to patients due to dose-limiting toxicity to the kidneys.
For imaging in head and neck cancer, tumour-to-tissue contrast of muscle, salivary glands, thyroid and, to some extent, bone is important, in addition to the tumour-to-blood ratio. Some normal mouse tissues containing squamous and transitional epithelium, such as tongue and skin, express murine CD44v6. However, we demonstrate in this study that AbD15179 does not cross-react with the murine CD44v6. Thus, studies with fragments cross-reactive with murine CD44v6 would be needed to study the effect of CD44v6 expression in normal tissues in a mouse model. Consequently, assuming that only non-CD44v6-mediated uptake was measured, our data indicate that there was no unspecific accumulation of 125I-Fab or 111In-Fab in any normal tissue. The transferability of the results to targeting HNSCC in humans is still somewhat limited, at least for organs with known CD44v6 expression as the epithelia of the oral cavity [17, 18]. However, previous clinical studies of CD44v6-binding antibodies have been performed for several CD44v6-tageting antibody conjugates for both radio-immunotherapy and radio-immunodiagnostics [23, 26, 45, 46], demonstrating no selective accumulation at non-tumour sites, except in faeces and urine , and only minimal accumulation of activity in mouth, lung, spleen, kidney, bone marrow and scrotal area . Since we have previously shown that AbD15179 at least partly binds to the same epitope as these antibodies , this is promising for the future clinical applicability of our fragment.
The isotopes that have physical and nuclear properties suitable for in vivo imaging use are relatively limited. In this study, we used 111In as a model for radiometals, and 125I as a model for radiohalogens. The choice of radionuclide and the labelling method is an important aspect in successful radio-immunotargeting, and additional improvements in tumour-to-blood ratio could probably be achieved by further optimizing or changing labelling methods or radionuclides. For 111In labelling, a CHX-A″-DTPA chelator-to-Fab molar ratio of 5:1 was chosen. It is possible that a different molar ratio could influence targeting properties of the conjugate. However, in a previous study, conjugation of 111In was studied at three different molar ratios (5:1, 10:1 and 20:1) of CHX-A″-DTPA chelate to panitumumab Fab2in vitro and in vivo. The different ratios had minimal effect on the immunoreactivity and specific binding . For radioiodination, direct CAT labelling was used, as it is a fast and simple method with high immunoreactive fraction yield, rendering high specific activity. 125I was used as a surrogate for radionuclides more suitable for imaging such as 124I, or 131I for therapy. The thyroid uptake was relatively high for 125I-Fab, most likely due to uptake of the free lipophilic iodine catabolites after proteolytic degradation of the antibody fragment. Likewise, the initially high uptake in salivary glands and stomach indicates iodine catabolites, since the Na+/I− symporter mediates active I− transport also in these tissues . Values in the same order have previously been reported for directly labelled 125I antibody conjugates [39, 48]. By using other labelling strategies or by blocking thyroid uptake using, e.g. potassium iodide, thyroid uptake may possibly be reduced .
Although intact monoclonal antibodies have been considered candidates for radio-immunodiagnostics due to their specificity, the long residence time in the blood, of days to weeks, limits their applicability. Several studies have demonstrated that the size of antibody-based imaging agents is inversely related to their blood clearance . Consequently, normal tissue exposure will be greater with the intact antibody than for the fragment, impairing tumour contrast. Fab fragments, approximately 50 kDa in size, have been developed predominantly as imaging agents. A possible disadvantage of Fab fragments is that their molecular weight subjects them to glomerular filtration. High uptake of antibody fragments in the kidneys has been reported before [49, 50], most likely a result of reabsorption by the renal tubular cells and catabolism . It is possible that with a larger format, such as Fab2 with a size above the renal threshold , the fragments would exhibit a biodistribution shift from the kidneys to the liver and may also improve affinity and tumour uptake even further. This will be assessed in a future study.
Another limitation of Fab fragments pertains to the fact that they are monovalent, often resulting in the loss of functional affinity and reduced binding strength compared to e.g. Fab2 or IgG. However, in vitro evaluations of our Fab fragment using SPR demonstrated KD in the low nanomolar range, which is considered optimal for imaging . Studies with 125I-labelled Fab fragments with lower affinity (KD 30 ± 8 nM) derived from the CD44v6-targeting chimeric antibody U36  have previously been performed in our group. The 125I-U36-Fab fragments demonstrated lower tumour uptake and lower tumour-to-blood ratios compared to the 125I-Fab in the present study, as well as less optimal tumour-to-organ ratios for e.g. salivary glands, tongue, thyroid and bone. The differences may be the result of many different aspects, for example, tumour cell line and size, as well as administered dose and specific activity of the conjugates. However, since AbD15179 was selected from a large number of fragments due to its high affinity and very slow dissociation from tumour cells , one hypothesis for the superior targeting qualities of 125I-Fab compared to those of 125I-U36-Fab may be the desirable kinetic properties of 125I-Fab. Thus, we believe that not only the fully human format but also the suitable affinity of AbD15179 will be an advantage for radio-immunodiagnostics.
We have, for the first time, evaluated the in vivo properties of the AbD15179 Fab fragment targeted against CD44v6 labelled with 111In or 125I and assessed its utility as a targeting agent for radio-immunotargeting of HNSCC. Our study demonstrated that 111In-Fab displayed a superior tumour-to-blood ratio in high-expressing CD44v6 tumours at all time points compared to 125I-Fab. Furthermore 111In-Fab was able to discriminate between moderate and high CD44v6 expression in vivo. We conclude that AbD15179 efficiently targets CD44v6-expressing squamous cell carcinoma xenografts, and particularly, the 111In-Fab displayed high and specific tumour uptake. CD44v6 emerges as a suitable target for radio-immunodiagnostics, and a fully human antibody fragment such as AbD15179 can enable further clinical imaging studies.
AKH is an otolaryngologist and head and neck surgeon, and a PhD student focusing on methods to detect squamous cell carcinoma in the head and neck region. DS has an MSc degree in Biology and is a PhD student focusing on molecular imaging and therapy of cancer. JN has a PhD degree in biotechnology and is currently a postdoctoral fellow working on antibody engineering. KS is an otolaryngologist and head and neck surgeon with a PhD degree in otorhinolaryngology. MN is an associate professor in Biomedical Radiation Sciences Unit, with a PhD degree in otorhinolaryngology.
bovine serum albumin
cluster of differentiation
v6 containing CD44
chimeric monoclonal antibody
Dulbecco's modified eagle medium
- Fab and Fab2:
fragment antigen binding
head and neck squamous cell carcinoma
human combinatorial antibody library
instant thin-layer chromatography
- K D :
equilibrium dissociation constant
gel filtration column
positron emission tomography
squamous cell carcinomas
single-photon emission tomography
surface plasmon resonance
monoclonal antibody U36.
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D: Global cancer statistics. CA Cancer J Clin 2011, 61: 69–90. 10.3322/caac.20107
Hammarstedt L, Lindquist D, Dahlstrand H, Romanitan M, Dahlgren LO, Joneberg J, Creson N, Lindholm J, Ye W, Dalianis T, Munck-Wikland E: Human papillomavirus as a risk factor for the increase in incidence of tonsillar cancer. Int J Cancer 2006, 119: 2620–2623. 10.1002/ijc.22177
Marklund L, Nasman A, Ramqvist T, Dalianis T, Munck-Wikland E, Hammarstedt L: Prevalence of human papillomavirus and survival in oropharyngeal cancer other than tonsil or base of tongue cancer. Cancer Med 2012, 1: 82–88. 10.1002/cam4.2
Lee CH, Wu DC, Lee JM, Wu IC, Goan YG, Kao EL, Huang HL, Chan TF, Chou SH, Chou YP, Lee CY, Chen PS, Ho CK, He J, Wu MT: Carcinogenetic impact of alcohol intake on squamous cell carcinoma risk of the oesophagus in relation to tobacco smoking. Eur J Cancer 2007, 43: 1188–1199. 10.1016/j.ejca.2007.01.039
Lopes CF, de Angelis BB, Prudente HM, de Souza BV, Cardoso SV, de Azambuja Ribeiro RI: Concomitant consumption of marijuana, alcohol and tobacco in oral squamous cell carcinoma development and progression: recent advances and challenges. Arch Oral Biol 2012, 57: 1026–1033. 10.1016/j.archoralbio.2012.05.006
Shah JP, Candela FC, Poddar AK: The patterns of cervical lymph node metastases from squamous carcinoma of the oral cavity. Cancer 1990, 66: 109–113. 10.1002/1097-0142(19900701)66:1<109::AID-CNCR2820660120>3.0.CO;2-A
Lindquist D, Romanitan M, Hammarstedt L, Nasman A, Dahlstrand H, Lindholm J, Onelov L, Ramqvist T, Ye W, Munck-Wikland E, Dalianis T: Human papillomavirus is a favourable prognostic factor in tonsillar cancer and its oncogenic role is supported by the expression of E6 and E7. Mol Oncol 2007, 1: 350–355. 10.1016/j.molonc.2007.08.005
Shreve PD, Anzai Y, Wahl RL: Pitfalls in oncologic diagnosis with FDG PET imaging: physiologic and benign variants. Radiographics 1999, 19: 61–77. quiz 150–151 10.1148/radiographics.19.1.g99ja0761
Olafsen T, Wu AM: Antibody vectors for imaging. Semin Nucl Med 2010, 40: 167–181. 10.1053/j.semnuclmed.2009.12.005
Yokota T, Milenic DE, Whitlow M, Schlom J: Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms. Cancer Res 1992, 52: 3402–3408.
Wong KJ, Baidoo KE, Nayak TK, Garmestani K, Brechbiel MW, Milenic DE: In vitro and in vivo pre-clinical analysis of a F(ab’)(2) fragment of panitumumab for molecular imaging and therapy of HER1 positive cancers. EJNMMI Res 2011, 1: 1. 10.1186/2191-219X-1-1
Sandstrom K, Haylock AK, Spiegelberg D, Qvarnstrom F, Wester K, Nestor M: A novel CD44v6 targeting antibody fragment with improved tumor-to-blood ratio. Int J Oncol 2012, 40: 1525–1532.
Milenic DE, Esteban JM, Colcher D: Comparison of methods for the generation of immunoreactive fragments of a monoclonal antibody (B72.3) reactive with human carcinomas. J Immunol Methods 1989, 120: 71–83. 10.1016/0022-1759(89)90291-3
Attarwala H: Role of antibodies in cancer targeting. J Nat Sci Biol Med 2010, 1: 53–56. 10.4103/0976-9668.71675
Chames P, Van Regenmortel M, Weiss E, Baty D: Therapeutic antibodies: successes, limitations and hopes for the future. Br J Pharmacol 2009, 157: 220–233. 10.1111/j.1476-5381.2009.00190.x
Heider KH, Kuthan H, Stehle G, Munzert G: CD44v6: a target for antibody-based cancer therapy. Cancer Immunol Immunother 2004, 53: 567–579. 10.1007/s00262-003-0494-4
Fox SB, Fawcett J, Jackson DG, Collins I, Gatter KC, Harris AL, Gearing A, Simmons DL: Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms. Cancer Res 1994, 54: 4539–4546.
Heider KH, Mulder JW, Ostermann E, Susani S, Patzelt E, Pals ST, Adolf GR: Splice variants of the cell surface glycoprotein CD44 associated with metastatic tumour cells are expressed in normal tissues of humans and cynomolgus monkeys. Eur J Cancer 1995, 31A: 2385–2391.
Orian-Rousseau V: CD44, a therapeutic target for metastasising tumours. Eur J Cancer 2010, 46: 1271–1277. 10.1016/j.ejca.2010.02.024
Fabricius EM, Guschmann M, Langford A, Hell B, Bier J: Immunohistochemical assessment of the tumour-associated epitopes CD44v6 and E48 in tumour-free lymph nodes from patients with squamous cell carcinoma in the head-neck region. Anal Cell Pathol 2000, 20: 115–129.
Verel I, Heider KH, Siegmund M, Ostermann E, Patzelt E, Sproll M, Snow GB, Adolf GR, van Dongen GA: Tumor targeting properties of monoclonal antibodies with different affinity for target antigen CD44V6 in nude mice bearing head-and-neck cancer xenografts. Int J Cancer 2002, 99: 396–402. 10.1002/ijc.10369
Kawano T, Nakamura Y, Yanoma S, Kubota A, Furukawa M, Miyagi Y, Tsukuda M: Expression of E-cadherin, and CD44s and CD44v6 and its association with prognosis in head and neck cancer. Auris Nasus Larynx 2004, 31: 35–41. 10.1016/j.anl.2003.09.005
Borjesson PK, Jauw YW, Boellaard R, de Bree R, Comans EF, Roos JC, Castelijns JA, Vosjan MJ, Kummer JA, Leemans CR, Lammertsma AA, van Dongen GA: Performance of immuno-positron emission tomography with zirconium-89-labeled chimeric monoclonal antibody U36 in the detection of lymph node metastases in head and neck cancer patients. Clin Cancer Res 2006, 12: 2133–2140. 10.1158/1078-0432.CCR-05-2137
Borjesson PK, Jauw YW, de Bree R, Roos JC, Castelijns JA, Leemans CR, van Dongen GA, Boellaard R: Radiation dosimetry of 89Zr-labeled chimeric monoclonal antibody U36 as used for immuno-PET in head and neck cancer patients. J Nucl Med 1828–1836, 2009: 50.
Colnot DR, Quak JJ, Roos JC, van Lingen A, Wilhelm AJ, van Kamp GJ, Huijgens PC, Snow GB, van Dongen GA: Phase I therapy study of 186Re-labeled chimeric monoclonal antibody U36 in patients with squamous cell carcinoma of the head and neck. J Nucl Med 1999–2010, 2000: 41.
Borjesson PK, Postema EJ, Roos JC, Colnot DR, Marres HA, van Schie MH, Stehle G, de Bree R, Snow GB, Oyen WJ, van Dongen GA: Phase I therapy study with (186)Re-labeled humanized monoclonal antibody BIWA 4 (bivatuzumab) in patients with head and neck squamous cell carcinoma. Clin Cancer Res 2003, 9: 3961S-3972S.
Stroomer JW, Roos JC, Sproll M, Quak JJ, Heider KH, Wilhelm BJ, Castelijns JA, Meyer R, Kwakkelstein MO, Snow GB, Adolf GR, van Dongen GA: Safety and biodistribution of 99mTechnetium-labeled anti-CD44v6 monoclonal antibody BIWA 1 in head and neck cancer patients. Clin Cancer Res 2000, 6: 3046–3055.
Nilvebrant J, Kuku G, Bjorkelund H, Nestor M: Selection and in vitro characterization of human CD44v6-binding antibody fragments. Biotechnol Appl Biochem 2012, 59: 367–380. 10.1002/bab.1033
Prassler J, Thiel S, Pracht C, Polzer A, Peters S, Bauer M, Norenberg S, Stark Y, Kolln J, Popp A, Urlinger S, Enzelberger M: HuCAL PLATINUM, a synthetic Fab library optimized for sequence diversity and superior performance in mammalian expression systems. J Mol Biol 2011, 413: 261–278. 10.1016/j.jmb.2011.08.012
Schrijvers AH, Quak JJ, Uyterlinde AM, van Walsum M, Meijer CJ, Snow GB, van Dongen GA: MAb U36, a novel monoclonal antibody successful in immunotargeting of squamous cell carcinoma of the head and neck. Cancer Res 1993, 53: 4383–4390.
Stenberg J, Spiegelberg D, Karlsson H, Nestor M: Choice of labeling and cell line influences interactions between the Fab fragment AbD15179 and its target antigen CD44v6. Nucl Med Biol 2013, 41(2):140–147.
Nestor M, Sundstrom M, Anniko M, Tolmachev V: Effect of cetuximab in combination with alpha-radioimmunotherapy in cultured squamous cell carcinomas. Nucl Med Biol 2011, 38: 103–112. 10.1016/j.nucmedbio.2010.06.014
Hunter WM, Greenwood FC: Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 1962, 194: 495–496. 10.1038/194495a0
Nestor M, Andersson K, Lundqvist H: Characterization of (111) In and (177)Lu-labeled antibodies binding to CD44v6 using a novel automated radioimmunoassay. J Mol Recognit 2008, 21: 179–183. 10.1002/jmr.883
Screaton GR, Bell MV, Bell JI, Jackson DG: The identification of a new alternative exon with highly restricted tissue expression in transcripts encoding the mouse Pgp-1 (CD44) homing receptor. Comparison of all 10 variable exons between mouse, human, and rat. J Biol Chem 1993, 268: 12235–12238.
Nestor M, Persson M, van Dongen GA, Jensen HJ, Lundqvist H, Anniko M, Tolmachev V: In vitro evaluation of the astatinated chimeric monoclonal antibody U36, a potential candidate for treatment of head and neck squamous cell carcinoma. Eur J Nucl Med Mol Imaging 2005, 32: 1296–1304. 10.1007/s00259-005-1848-2
Brown BA, Comeau RD, Jones PL, Liberatore FA, Neacy WP, Sands H, Gallagher BM: Pharmacokinetics of the monoclonal antibody B72.3 and its fragments labeled with either 125I or 111In. Cancer Res 1987, 47: 1149–1154.
Sandstrom K, Nestor M, Ekberg T, Engstrom M, Anniko M, Lundqvist H: Targeting CD44v6 expressed in head and neck squamous cell carcinoma: preclinical characterization of an 111In-labeled monoclonal antibody. Tumour Biol 2008, 29: 137–144. 10.1159/000143399
Fortin MA, Salnikov AV, Nestor M, Heldin NE, Rubin K, Lundqvist H: Immuno-PET of undifferentiated thyroid carcinoma with radioiodine-labelled antibody cMAb U36: application to antibody tumour uptake studies. Eur J Nucl Med Mol Imaging 2007, 34: 1376–1387. 10.1007/s00259-006-0346-5
Ahl PL, Bhatia SK, Meers P, Roberts P, Stevens R, Dause R, Perkins WR, Janoff AS: Enhancement of the in vivo circulation lifetime of L-alpha-distearoylphosphatidylcholine liposomes: importance of liposomal aggregation versus complement opsonization. Biochim Biophys Acta 1997, 1329: 370–382. 10.1016/S0005-2736(97)00129-6
Khaw BA, Strauss HW, Cahill SL, Soule HR, Edgington T, Cooney J: Sequential imaging of indium-111-labeled monoclonal antibody in human mammary tumors hosted in nude mice. J Nucl Med 1984, 25: 592–603.
Behr TM, Becker WS, Sharkey RM, Juweid ME, Dunn RM, Bair HJ, Wolf FG, Goldenberg DM: Reduction of renal uptake of monoclonal antibody fragments by amino acid infusion. J Nucl Med 1996, 37: 829–833.
Behr TM, Goldenberg DM, Becker W: Reducing the renal uptake of radiolabeled antibody fragments and peptides for diagnosis and therapy: present status, future prospects and limitations. Eur J Nucl Med 1998, 25: 201–212. 10.1007/s002590050216
Behr TM, Sharkey RM, Juweid ME, Blumenthal RD, Dunn RM, Griffiths GL, Bair HJ, Wolf FG, Becker WS, Goldenberg DM: Reduction of the renal uptake of radiolabeled monoclonal antibody fragments by cationic amino acids and their derivatives. Cancer Res 1995, 55: 3825–3834.
Postema EJ, Borjesson PK, Buijs WC, Roos JC, Marres HA, Boerman OC, de Bree R, Lang M, Munzert G, van Dongen GA, Oyen WJ: Dosimetric analysis of radioimmunotherapy with 186Re-labeled bivatuzumab in patients with head and neck cancer. J Nucl Med 2003, 44: 1690–1699.
Colnot DR, Roos JC, de Bree R, Wilhelm AJ, Kummer JA, Hanft G, Heider KH, Stehle G, Snow GB, van Dongen GA: Safety, biodistribution, pharmacokinetics, and immunogenicity of 99mTc-labeled humanized monoclonal antibody BIWA 4 (bivatuzumab) in patients with squamous cell carcinoma of the head and neck. Cancer Immunol Immunother 2003, 52: 576–582. 10.1007/s00262-003-0396-5
Portulano C, Paroder-Belenitsky M, Carrasco N: The Na(+)/I(−) symporter (NIS): mechanism and medical impact. Endocr Rev 2014, 35: 106–149. 10.1210/er.2012-1036
Nestor M, Persson M, Cheng J, Tolmachev V, van Dongen G, Anniko M, Kairemo K: Biodistribution of the chimeric monoclonal antibody U36 radioiodinated with a closo-dodecaborate-containing linker. Comparison with other radioiodination methods. Bioconjug Chem 2003, 14: 805–810. 10.1021/bc034003n
Khawli LA, Alauddin MM, Hu P, Epstein AL: Tumor targeting properties of indium-111 labeled genetically engineered Fab’ and F(ab’)2 constructs of chimeric tumor necrosis treatment (chTNT)-3 antibody. Cancer Biother Radiopharm 2003, 18: 931–940. 10.1089/108497803322702897
Massuger LF, Boerman OC, Corstens FH, Verheijen RH, Claessens RA, Poels LG, van den Broek WJ, Kenemans P: Biodistribution of iodine-125 and indium-111 labeled OV-TL 3 intact antibodies and F(ab’)2 fragments in tumor-bearing athymic mice. Anticancer Res 2051–2057, 1991: 11.
Wochner RD, Strober W, Waldmann TA: The role of the kidney in the catabolism of Bence Jones proteins and immunoglobulin fragments. J Exp Med 1967, 126: 207–221. 10.1084/jem.126.2.207
Holechek MJ: Glomerular filtration: an overview. Nephrol Nurs J 2003, 30: 285–290. quiz 291–282
Rudnick SI, Adams GP: Affinity and avidity in antibody-based tumor targeting. Cancer Biother Radiopharm 2009, 24: 155–161. 10.1089/cbr.2009.0627
The authors would like to acknowledge the Swedish Cancer Society, the Swedish Association for Medical Research and Selanders Foundation for their kind support. The authors would also like to thank Anja Mortensen and Hampus Karlsson for their help with the in vitro characterizations. JN acknowledges the support from the Hans Werthén Foundation.
The authors declare that they have no competing interests.
AKH contributed to the conception and design of the study, carried out the experimental studies and wrote the manuscript. DS, JN, KS and MN contributed to the conception and design of the study, carried out the experimental studies and critically contributed to and revised the manuscript. All authors read and approved the final manuscript.
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Haylock, AK., Spiegelberg, D., Nilvebrant, J. et al. In vivo characterization of the novel CD44v6-targeting Fab fragment AbD15179 for molecular imaging of squamous cell carcinoma: a dual-isotope study. EJNMMI Res 4, 11 (2014). https://doi.org/10.1186/2191-219X-4-11
- Antibody fragment
- Molecular imaging