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Table 2 Absolute ABCB1, ABCG2 and ATPase levels in plasma membrane fractions of surgically removed tumor tissue determined with quantitatively targeted absolute proteomics

From: Assessment of brain delivery of a model ABCB1/ABCG2 substrate in patients with non-contrast-enhancing brain tumors with positron emission tomography

 Total protein used for digestion (μg) (n)ABCB1
(fmol/μg) (CV%)
ABCG2
(fmol/μg) (CV%)
ATPase
(fmol/μg) (CV%)
p0150 (1)0.97 (<5%)1.53 (<5%)178.3 (<5%)
p0250 (4)0.79 (2.7 %)0.85 (4.4 %)257.1 (0.6%)
p03a25 (1)0.69 (<5%)0.94 (<5%)60.1 (<5%)
p03a50 (3)0.94 (6.4 %)1.4 (8.2 %)164.6 (7.1 %)
p0540 (1)0.64 (<5%)1.10 (<5%)83.3 (<5%)
  1. For lower limits of quantification, see [27]
  2. %CV, % coefficient of variation: n = digestion replicate, each digested sample was injected three times. Quantification CV% <5% is the variation between transitions and results from three injections (for the three samples digested once and injected three times)
  3. aTwo different samples collected during surgery