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EJNMMI Research

Table 2 Absolute ABCB1, ABCG2 and ATPase levels in plasma membrane fractions of surgically removed tumor tissue determined with quantitatively targeted absolute proteomics

From: Assessment of brain delivery of a model ABCB1/ABCG2 substrate in patients with non-contrast-enhancing brain tumors with positron emission tomography

 

Total protein used for digestion (μg) (n)

ABCB1

(fmol/μg) (CV%)

ABCG2

(fmol/μg) (CV%)

ATPase

(fmol/μg) (CV%)

p01

50 (1)

0.97 (<5%)

1.53 (<5%)

178.3 (<5%)

p02

50 (4)

0.79 (2.7 %)

0.85 (4.4 %)

257.1 (0.6%)

p03a

25 (1)

0.69 (<5%)

0.94 (<5%)

60.1 (<5%)

p03a

50 (3)

0.94 (6.4 %)

1.4 (8.2 %)

164.6 (7.1 %)

p05

40 (1)

0.64 (<5%)

1.10 (<5%)

83.3 (<5%)

  1. For lower limits of quantification, see [27]
  2. %CV, % coefficient of variation: n = digestion replicate, each digested sample was injected three times. Quantification CV% <5% is the variation between transitions and results from three injections (for the three samples digested once and injected three times)
  3. aTwo different samples collected during surgery
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