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Table 1 HPLC methods

From: Design, synthesis and evaluation in an LPS rodent model of neuroinflammation of a novel 18F-labelled PET tracer targeting P2X7

Method EFB Rt (min) Flow rate (mL/min) Column Mobile phase gradient Mobile phase UV (nm)
A 51’10” 3 Agilent eclipse XDB C18, 9.4 × 250 mm, 5 μm, 100 Å 15% B for 5 min; 15–35% B for 1 h; 35% B for 2 min; 35–20% B for 2 min; 20% B for 1 min A: H2O 254
B: MeCN
B 19’10” 1 Kinetex Phenomenex XB-C18 with guard, 4.6 × 150 mm, 5 μm, 100 Å 30 s at 20% B; 23.5 min 20–33.5% B; 3 min 33.5–70% B; 2 min 70% B; 1 min 70–20% B A: H2O 300
B: MeCN