Design, synthesis and evaluation in an LPS rodent model of neuroinflammation of a novel 18F-labelled PET tracer targeting P2X7

EJNMMI Research

Table 1 HPLC methods

Method	EFB Rt (min)	Flow rate (mL/min)	Column	Mobile phase gradient	Mobile phase	UV (nm)
A	51’10”	3	Agilent eclipse XDB C18, 9.4 × 250 mm, 5 μm, 100 Å	15% B for 5 min; 15–35% B for 1 h; 35% B for 2 min; 35–20% B for 2 min; 20% B for 1 min	A: H2O	254
A	51’10”	3	Agilent eclipse XDB C18, 9.4 × 250 mm, 5 μm, 100 Å		B: MeCN	254
B	19’10”	1	Kinetex Phenomenex XB-C18 with guard, 4.6 × 150 mm, 5 μm, 100 Å	30 s at 20% B; 23.5 min 20–33.5% B; 3 min 33.5–70% B; 2 min 70% B; 1 min 70–20% B	A: H2O	300
B	19’10”	1			B: MeCN	300