Figure 4

Immunostaining for TNC at 3, 7, and 14 days after reperfusion with and without postconditioning is presented. Strong TNC brown staining was seen in border zone between intact and infarcted area, and also scattered brown staining was seen throughout the infarcted area at 3 days after reperfusion. With postconditioning, TNC staining tended to be weaker in both border zone and infarcted area. At 7 and 14 days after reperfusion, TNC staining became weaker at both border zone and infarcted area. With postconditioning, TNC staining at both border zone and infarcted area became weaker. Intact, intact area; Inf, infarcted area; Scale bars, 100 μm.