Figure 7

Autoradiography, immunofluorescence, immunohistochemistry, and overlay images. (A) Autoradiography image from a section of NIH:OVCAR-3 tumor indicating hot spots of in vivo targeting by ⁶⁴Cu-labeled anti-CA125 MAb. (B) Immunofluorescence image of the same section staining green in regions of CA125 expression (Alexa Fluor 488) and counterstained blue (Hoechst 33342) for nuclei. (C) Overlay of images (A) and (B) to establish concurrence between signals from independent methods of ex vivo analysis to indicate CA125 expression and targeting. (D) Autoradiography image from a separate section of NIH:OVCAR-3 tumor indicating hot spots of in vivo targeting by ⁶⁴Cu-labeled anti-CA125 MAb. (E) Immunohistochemistry image of corresponding tissue section. Intensity of staining is indicative of CA125 expression across the sample. (F) Overlay of images (D) and (E) to establish concurrence between signals from independent methods of ex vivo analysis to indicate CA125 expression and targeting.