Fig. 3

[⁸⁹Zr]-DFO-RW03_scFv-Fc ImmunoPET imaging CD133 expressing tumors. [⁸⁹Zr]-DFO(2.9)-RW03_scFv-Fc PET imaging study (a & c) and corresponding ROI analysis (b & d). Mice received (a & b) [⁸⁹Zr]-DFO(2.9)-RW03_scFv-Fc (1.42 ± 0.07 MBq, 16.0 ± 0.2 ug) or (c & d) [⁸⁹Zr]-DFO(2.9)-RW03_scFv-Fc (1.40 ± 0.09 MBq, 16.0 ± 0.3 ug) co-injected with RW03_IgG (0.5 mg). For PET images, the transverse and coronal planes are presented as maximum intensity projections (MIP). White arrow = tumor. For ROI analysis (b, d) uptake is expressed as mean radioactivity per mL tissue volume (%ID/cc) ± SEM