Glial reactivity in a mouse model of beta-amyloid deposition assessed by PET imaging of P2X7 receptor and TSPO using [11C]SMW139 and [18F]F-DPA

EJNMMI Research

Table 1 Mice used in ex vivo studies

	Age (mo)	Genotype	N_GENDER		N_TOTAL
	Age (mo)	Genotype	F	M	N_TOTAL
Ex vivo brain autoradiography	5	TG	3	1	4
		WT	2	2	4
	8–10	TG	3	2	5
		WT	2	5	7
	12	TG	–	2	2
		WT	–	1	1
	14	TG	1	1	2
		WT	3	2	5
P2X7 immunohistochemical staining	5	TG	1	–	1
		WT	–	1	1
	8–10	TG	1	1	2
		WT	–	2	2
	12	TG	–	2	2
		WT	–	2	2
	14	TG	1	–	1
		WT	1	–	1
	15	TG	1	–	1
		WT	–	–	–
TSPO & P2Y12 immunohistochemical staining	5	TG	2	1	3
		WT	1	2	3
	8–10	TG	2	1	3
		WT	1	2	3
	12	TG	–	3	3
		WT	–	2	2
	14	TG	2	1	3
		WT	4	–	4
	15	TG	1	2	3
		WT	–	–	–

	Δt (min)	Genotype	N_GENDER		N _TOTAL
	Δt (min)	Genotype	F	M	N _TOTAL
Plasma radiometabolites analysis	10	TG	4	9	13
		WT	8	8	16
	30	TG	3	–	3
		WT	5	8	13
	45	TG	3	–	3
		WT	9	2	11
Brain radiometabolites analysis	10	TG	4	2	6
		WT	7	3	10
	30	TG	3	–	3
		WT	5	9	14
	45	TG	3	–	3
		WT	9	1	10