Fig. 6

Immunohistochemical staining of P2X7 receptor in brain cryosections of APP/PS1-21 transgenic (TG) and wild type (WT) mice. a Quantification of P2X7-positive staining as object counts/mm². Staining was evaluated in the cortex and thalamus of TG and WT mice at 5, 8–10, 12, 14, and 15 months. b Representative images of P2X7-positive staining in TG and WT mice at the investigated time points. Arrows point at the P2X7-positive staining detected by the artificial intelligence object detection algorithm. Scale bar = 1000 µm for the half-hemisphere brain section image, 100 µm for the cortex view image. Magnification = 1.5× for the half-hemisphere brain section image, 15.0 × for the cortex view image