Fig. 5

Examples of endometriotic lesions identified using H&E (top) and the corresponding areas stained with fluorescent markers. Arrowheads identify endometriotic tissue regions. Cell nuclei are stained with DAPI (blue), Fn is depicted in green and relaxed Fn (Cy5-FnBPA5) in pink. a An endometriotic lesion containing endometriosis stroma and epithelium with characteristic Cy5-FnBPA5 staining. b Same specimen as in (A), but another endometriotic region. Particularly, strong Cy5-FnBPA5 staining is observed in a region displaying reactive fibrosis (RF). c Disrupted cyst (Cy) lined by endometriotic epithelium. FnBPA5 shows strong signal in the proximity of the cyst, but spares the direct border of the cyst, which is lined by endometriotic epithelium. d A region of endometriotic stroma that displays especially strong staining for relaxed Fn. e Close-up on a characteristic endometriotic gland lined by endometriotic epithelium both by H&E and using Cy5-FnBPA5. Cy5-FnBPA5 is not staining the epithelium. f FnBPA5 staining in regions of thermal ablation caused as an artifact from surgical removal