Fig. 3

[³H]NS14490 autoradiography following MI reveals high α7nAChR expression within the infarct and border region at day 14 and day 28 following ischemia and reperfusion injury. A Representative H&E images of the heart from sham (non-infarcted) and MI animals from day 2 to day 28, scale bar = 1 mm. B Corresponding [³H]NS14490 autoradiography images showing total binding and blocked (10 µM NS14490) binding examples. C Quantification of the autoradiography specific binding signal across the left ventricle, with D showing quantification in the infarct, E infarct border and F remote inferoseptal region. N = 4–6, * = P ≤ 0.05, ** = P ≤ 0.01 and **** = P ≤ 0.0001 using two-way ANOVA with post hoc Šidák’s test for sham versus MI.