Table 2 Details of primary antibodies used for immunohistochemistry
Target | Dilution | Supplier | Reference |
|---|---|---|---|
CA-IX | 1:350 | Novus Biologicals | NB 100-417 |
HIF-1α | 1:500 | Cell signaling | #36,169 |
HIF-2α | 1:250 | GeneTex | #30,114 |
DMT1 | 1:200 | Abcam | Ab55735 |
CTR-1 | 1:500 | Novus Biologicals | NB 100-402 |
Pimonidazole | 1:200 | Hypoxyprobe Inc. | HP7-1000Kit |
GFAP | 1:200 | DAKO | Z0334 |