Heterogeneity of prostate-specific membrane antigen (PSMA) and PSMA-ligand uptake detection combining autoradiography and postoperative pathology in primary prostate cancer

EJNMMI Research

Table 2 Immunoreactive and autoradiographic-reactive sore

Immunoreactive score (IRS) *
Intensity of staining (membrane)	Percentage of positive cells	IRS^#
0 = no color reaction	0 = no positive cells	0–1 = negative
1 = mild reaction	1 = < 10% positive cells	2–3 = mild
2 = moderate reaction	2 = 10%–50% positive cells	4–8 = moderate
3 = intense reaction	3 = 51%–80% positive cells	9–12 = strong
	4 = > 80%positive cells

Autoradiographic-reactive score (ARS)
Intensity of signal	Percentage of positive grids	ARS^§
0 = < 0.5*average SUV of non-tumor grids	0 = no positive grids	0–1 = negative
1 = 1st tri-sectional quantile of the rest SUV	1 = < 10% positive grids	2–3 = mild
2 = 2nd tri-sectional quantile of the rest SUV	2 = 10%–50% positive grids	4–8 = moderate
3 = 3rd tri-sectional quantile of the rest SUV	3 = 51%–80% positive grids	9–12 = strong
	4 = > 80%positive cells

^*Modified from Woythal and Kaemmerer et al. (41, 42)
^#\(\mathrm{IRS}=\mathrm{Intensity of staining}\times \mathrm{Percentage of positive cells}\)
^§ \(\mathrm{ARS}=\mathrm{Intensity of signal}\times \mathrm{Percentage of positive grids}\). Please note that the ARS is based on grids whereas the IRS takes into account the percentage of positive cells