Fig. 5

Histological assessment of VLA-4 expression in murine lungs with bleomycin-induced fibrosis. Representative low-magnification (top panels) and high-magnification (bottom panels) images demonstrate a marked increase in the binding of LLP2A-Cy3 (red) in fibrotic lungs of the mice at 4 weeks after bleomycin administration compared to the control lungs. The specificity of LLP2A-Cy3 binding is confirmed by the blocking of LLP2A-Cy3 signal in tissues co-incubated with excess non-fluorescent LLP2A (the insets in the right lower corners of high-magnification images). Tissues are counterstained with DAPI nuclear staining (blue). N = 3 per group