In vitro evaluation of (S)-2-amino-3-[3-(2-18F-fluoroethoxy)-4-iodophenyl]-2-methylpropanoic acid (18F-FIMP) as a positron emission tomography probe for imaging amino acid transporters

EJNMMI Research

Table 2 Knockdown efficiency at the protein level

	LAT1	ATB^0,+	ASCT2	xCT
Transfected cells	0	0	0	0.09
	(546/546)	(214/214)	(258/258)	(198/217)
Transfected cells + siRNA	0.92	0.99	0.93	0.98
	(91/1105)	(5/1284)	(77/1156)	(21/1353)

Knockdown efficiency = 1−[(No. of immuno-positive cells)/(No. of total cells)]
siRNA, small interfering ribonucleic acid; No., number; LAT1, L-type amino acid transporter 1; ATB^0,+, sodium- and chloride-dependent neutral and basic amino acid transporter B(0 +); ASCT2, alanine serine cysteine transporter 2; xCT, cystine/glutamate transporter