Fig. 2
In vitro characterization of [99mTc]Tc-N4-PSMA-12, [99mTc]Tc-N4-PSMA-13, [99mTc]Tc-N4-PSMA-21 and [99mTc]Tc-PSMA-I&S: A Binding affinity to PSMA (IC50,inv. (nM), 1 h, 4 °C, n = 3); B PSMA-mediated internalization (1 h, 37 °C, (% of the reference [.125I]IBA-KuE), n = 3); C lipophilicity expressed as logD7.4 (n-octanol/PBS, pH 7.4, n = 8); D binding to human plasma (PPB) (incubation at 37 °C for 30 min, determination via ultrafiltration (%), n = 6)