Fig. 4
From: Promise and challenges of clinical non-invasive T-cell tracking in the era of cancer immunotherapy
Schematic representation of direct T-cell labelling imaging approach. After blood sampling, white blood cells (WBCs) are isolated from peripheral blood mononuclear cells (PBMCs) via density-gradient centrifugation, and incubated with [99mTc]Tc-HMPAO (shown in the panel) or alternatively with [111In]In-oxine. The radiolabelled WBCs are then re-infused in patients and their homing in inflamed organs visualized via single photon emission tomography (SPECT) imaging