Fig. 2

a Kaplan–Meier analysis of glycan fragments in cancer region. Blue lines indicate survival in patients with high intensity of the respective mass. Red lines indicate survival in patients with low intensity of the respective mass. Example Kaplan–Meier curves of 4 good prognosis and 4 poor prognosis glycan masses in cancer cell region are shown. In the upper panel, a high abundance of Hex-HexNAcS (m/z 462.0937), Hex-HexNAcS-HexNAc (m/z 665.1725), dHex-HexS (m/z 405.0710) and NeuAc-Hex-HexNAcP (m/z 753.1975) in cancer cell regions was associated with good prognosis. In contrast, in lower panel, high abundance of HexA (m/z 193.0350), chondroitin/hyaluronan (m/z 378.1050), dHex-Hex-HexAMe (m/z 515.1625) and (m/z 599.1955) HexNAc-HexA-HexNAc was associated with poor patient prognosis. b Kaplan–Meier analysis of glycan fragments in the stroma region. Blue lines indicate survival in patients with high intensity of the respective mass. Red lines indicate survival in patients with low intensity of the respective mass. High abundance of HexS (m/z 259.0135) in stroma regions was associated with good prognosis. High abundance of HexNAcS (m/z 282.0290), HexA-HexNAc (m/z 396.1150), HexNAcSS (m/z 401.9785) and HexA-HexNAcS (m/z 476.0725) in stroma regions was associated with poor prognosis