Fig. 2
From: In vitro proof of concept studies of radiotoxicity from Auger electron-emitter thallium-201
201Tl kinetics. a Uptake of 201Tl in DU145 cells (n = 3) after incubation with 200 kBq/mL [201Tl]TlCl in medium. These results were used to determine the best incubation time for subsequent radiobiological experiments. b 201Tl uptake in DU145 cells (n = 3) and MDA-MD-231 cells (n = 2) after 90 min incubation in medium with different activities of [201Tl]TlCl. c 201Tl intracellular activity per single cell (Bq) versus activity added (kBq/mL) in DU145 cells (n = 3) measured in medium. Graphs b and c show that the increasing activity of 201Tl does not inhibit its cellular uptake, suggesting that saturation of uptake mechanisms is not reached at the activities used in this work. d Efflux of 201Tl in DU145 (n = 3) cells. Plateau uptake from the preceding uptake assay is defined as 100% at time 0. Radioactive medium was replaced with non-radioactive and 201Tl activity was measured over time. Data are presented as mean ± SD, done in triplicates; exponential regression line was fitted in d. Some error bars are smaller than the data symbols and hence not visible