Fig. 3
From: Intraoperative visualization of nerves using a myelin protein-zero specific fluorescent tracer
Staining of 3D DRG explant cultures with Cy5-P0101–125 and Cy5-P0101–125 fluorescence immunohistochemistry of a murine nervus ischiadicus. A brightfield image of a 3D DRG explant (*) with axonal outgrowths (white arrow) after staining with (II) Cy5-P0101–125 or (III) Cy5-P0ex. B Zoom-in with focus on the axonal outgrowths and fluorescence confocal imaging of DRG explants after staining with (II) Cy5-P0101–125 or (III) Cy5-P0ex. C Fluorescence immunohistochemistry of the nervus ischiadicus of THY1-YFP mice in (I) transverse orientation and (II) sagittal orientation after staining with Cy5-P0101–125. The top insert shows standard anti-body-based standard immunohistochemistry and the bottom insert H&E staining of a concurrent/adjacent tissue section