Conscious rat PET imaging with soft immobilization for quantitation of brain functions: comprehensive assessment of anesthesia effects on cerebral blood flow and metabolism

EJNMMI Research

Table 1 Blood glucose levels and plasma corticosterone levels of conscious and anesthetized rats

	Blood glucose levels (mmol/L)	Plasma corticosterone levels (ng/mL)
	Before [¹⁸F]FDG injection	Before [¹⁸F]FDG injection	After [¹⁸F]FDG-PET	After [¹²⁵I]IMP assay
Conscious	7.51 ± 8.1	10.4 ± 5.0	13.6 ± 3.4^†	15.9 ± 3.2^††
MMB	10.0 ± 2.5**	9.42 ± 5.98	2.87 ± 3.59**	2.90 ± 4.79**
KX	10.6 ± 0.4**	n.d	n.d	n.d
Chloral	7.99 ± 1.35	n.d	n.d	n.d
PTB	6.30 ± 0.56	n.d	n.d	n.d
PF	6.42 ± 0.77	n.d	n.d	n.d
IFL	7.73 ± 0.81	8.94 ± 3.17	5.44 ± 2.66*	6.64 ± 3.96*

Data are expressed as mean ± SD (n = 6–7 for blood glucose levels, and n = 4 for plasma corticosterone levels). *P < 0.05 and **P < 0.01 compared with conscious rats as determined using ANOVA with Dunnett’s multiple comparison test. ^†P < 0.05 and ^††P < 0.01 compared with before [¹⁸F]FDG injection as determined using repeated ANOVA with Tukey’s multiple comparison test. n.d. not determined