Fig. 4
Images of tumor histology and autoradiography data showing distribution of 64Cu-SARTATE in tumor deposits and surrounding liver. Top row a shows a hematoxylin and eosin-stained frozen section of liver tissue (pink) containing darker purple tumor deposits and its corresponding autoradiograph (b) (size scale bar bottom left of a, autoradiography data scale bar right of b). Details of regions indicated by lower case a–d are shown in figure. Middle row c shows a contiguous section that has been stained using immunohistochemistry to demonstrate SSTR2 expression (dark brown) counter-stained with hematoxylin (blue) and its autoradiograph (d). Uptake of the radiolabeled peptide was highest in regions of tumor that were both viable and stained strongly for the target SSTR2. Bottom row (e) shows a surface plot of the autoradiograph B generated in ImageJ showing the relative uptakes in liver, viable SSTR2-positive tumor and necrotic tumor. Uptake in the small group of cells surrounded by liver “c” and the small SSTR2-positive deposit “d” are indicated with arrows for orientation