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Fig. 4 | EJNMMI Research

Fig. 4

From: Indium-111-labeled CD166-targeted peptide as a potential nuclear imaging agent for detecting colorectal cancer stem-like cells in a xenograft mouse model

Fig. 4

Screening and identification of CD166-targeted peptide. The phage display (12 phage library) was used to screen CD166 binding phages with four rounds of biopanning. a The titers of recovered phages from each round of biopanning were eluted by a blue/white colony screening on LB/IPTG/Xgal plate. The phage enrichment rate was calculated as output number/input number. b Phage ELISA assay. The carrying different peptide sequences of phage clones were exposed to CD166 protein and BSA (as a control) for detecting the binding affinity. c Phage binding to CD166 expression cells. The phage clones were further screened by cell-based phage ELISA. The CD166+HCT15 and CD166HCT15 cells were positive and negative CD166 protein expression cells, respectively. Data are presented as mean ± SD (n ≥ 3)

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