Fig. 1

PD-L1 total protein expression analysis of four CRC cell lines. a Western blot analysis of total cell lysates using anti-PD-L1 and anti-β-actin antibody between LoVo, LS174T, SW620, and RKO in vitro. b The ratio of PD-L1 total protein intensity. Data are expressed as means ± SD, *** p < 0.001 (n = 3)