Fig. 1
From: Fluorescence labeling of a NaV1.7-targeted peptide for near-infrared nerve visualization
Ion channel selectivity and chemical synthesis of Hs1a-FL. a Representative view of the experimental settings. A 3D rendering of a frozen and sliced mouse. White arrows show the left sciatic nerve. Right sciatic nerve magnification shows the fluorescent Hs1a-FL agent bound to the nerve surface. b Selectivity of Hs1a towards human NaV channels stably expressed in HEK293 cells. Calculated IC50 values were hNaV1.1; 19.4 nM, hNaV1.2; 81.2 nM, hNaV1.3; 106.8 nM, hNaV1.4; > 3000 nM, hNaV1.5; > 3000 nM, hNaV1.6; 19.2 nM, hNaV1.7; 26.9 nM. Each point on the curve is an average of 3–11 cells. c Reaction scheme for conjugation of Hs1a peptide with Cyanine7.5-NHS ester dye. The ribbon model of Hs1a-FL shows disulfide bridges (in yellow) and shows the attachment of one dye to the peptide (orange/blue)