Table 3 Apoptosis analysis of MDA-MB-231(a) and MDA-MB-468(b) cells after incubation with treatment at 10xIC50 for 24 h, 48 h, and 72 h by flow cytometry using Annexin V/PI apoptosis assay. Results are shown as mean ± SD (n = 3 independent experiments)
MDA-MB-468 | |||||||
| Â | Untreated | Epirubicin | Paclitaxel | ||||
| Â | 24 h | 48 h | 72 h | 72 h | 24 h | 48 h | 72 h |
Live cells (%) | 88.7 ± 1.4 | 83.3 ± 2.4 | 83.8 ± 2.3 | 30.1 ± 0.9 | 67.6 ± 2.5 | 45.3 ± 3.2 | 23.0 ± 1.6 |
Apoptotic cells (%) | 6.8 ± 1.1 | 8.6 ± 2.3 | 8.8 ± 0.7 | 49.5 ± 1.2 | 23.6 ± 1.5 | 38.8 ± 2.8 | 53.3 ± 4.2 |
Necrotic cells (%) | 4.5 ± 1.6 | 8.1 ± 3.0 | 7.3 ± 2.7 | 20.4 ± 0.3 | 8.8 ± 2.0 | 15.9 ± 3.7 | 23.8 ± 4.3 |