Fig. 4

Characterization of apoptotic tumor after treatment by paclitaxel in MDA-MB-468 subcutaneously xenograft model. a–c Western blot analysis showing the cleavage of PARP and Caspase 3 on tumors samples. Actin was used as a loading control. Individual bands (M1, M2, M3) correspond to 2–3 independent tumor samples per group of treatment. b–d Quantification of cleaved PARP as a ratio to PARP. Data are presented as the mean ± SD. Mann-Whitney test was performed (*p < 0.05). Significant difference versus control group. e Increase of cleaved caspase 3-positive tumor cells was observed after paclitaxel treatment. f Quantitative analysis of apoptotic cells measured by flow cytometry in control or paclitaxel treated. Data are presented as the mean ± SD. Compared to control, ***p < 0.001 (Mann-Whitney test)