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Table 1 Binding affinities (IC50 in nM) of the reference ligands FC-131 and Ga-pentixafor as well as various CPCR4.3 and iodo-CPCR4.3 to human and mouse CXCR4 (hCXCR4, mCXCR4). Affinities were determined using Jurkat human T cell leukemia and Eμ-Myc1080 mouse B cell lymphoma cells, respectively (200,000 cells/sample), and [125I]CPCR4.3 as the radioligand. Each experiment was performed in triplicate, and results are means ± SD from three separate experiments

From: Validation of [125I]CPCR4.3 as an investigative tool for the sensitive and specific detection of hCXCR4 and mCXCR4 expression in vitro and in vivo

Peptide IC50 [nM] to hCXCR4 IC50 [nM] to mCXCR4
FC-131 12.4 ± 3.1 119 ± 69
Ga-Pentixafor 42.4 ± 11.6 > 1000
CPCR4.3 2.8 ± 1.1 0.8 ± 0.1
iodo-CPCR4.3 5.4 ± 1.5 4.9 ± 1.7