Table 1 Binding affinities (IC₅₀ in nM) of the reference ligands FC-131 and Ga-pentixafor as well as various CPCR4.3 and iodo-CPCR4.3 to human and mouse CXCR4 (hCXCR4, mCXCR4). Affinities were determined using Jurkat human T cell leukemia and Eμ-Myc1080 mouse B cell lymphoma cells, respectively (200,000 cells/sample), and [¹²⁵I]CPCR4.3 as the radioligand. Each experiment was performed in triplicate, and results are means ± SD from three separate experiments

From: Validation of [¹²⁵I]CPCR4.3 as an investigative tool for the sensitive and specific detection of hCXCR4 and mCXCR4 expression in vitro and in vivo