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Table 2 In vitro tumor cell binding characteristics: determination of dissociation constants (Kd) in nM and apparent number of binding sites per cell (Bmax) of the 68Ga-labeled BN peptides (n = 3-5 experiments; mean values ± SD; Bmax SD < 15%)

From: A convenient and efficient total solid-phase synthesis of DOTA-functionalized tumor-targeting peptides for PET imaging of cancer

  68Ga-DOTA-Ahx-BN 68Ga-DOTA-Abz-BN 68Ga-DOTA-ALA-BN
Cyclen Tris- Cyclen Tris- Cyclen Tris-
MDA K d 12.73 ± 2.94 11.60 ± 3.05 11.81 ± 2.11 13.10 ± 1.29 15.14 ± 3.12 14.68 ± 2.24
B max 5.13 × 105 5.27 × 105 6.09 × 105 5.89 × 105 1.02 × 105 1.12 × 105
MCF7 K d 9.80 ± 1.64 9.32 ± 1.50 10.75 ± 1.85 10.98 ± 1.10 13.69 ± 2.32 12.30 ± 1.94
B max 6.90 × 105 6.22 × 105 5.12 × 105 4.86 × 105 2.11 × 105 1.05 × 105
T47D K d 15.28 ± 3.42 14.51 ± 2.40 15.04 ± 3.24 13.82 ± 2.66 15.56 ± 3.47 16.0 ± 4.01
B max 4.75 × 105 4.91 × 105 1.17 × 105 1.0 × 105 9.90 × 104 9.68 × 104
PC3 K d 9.89 ± 1.56 10.45 ± 1.71 12.52 ± 2.09 11.24 ± 2.17 9.70 ± 2.01 13.49 ± 2.53
B max 5.29 × 105 4.88 × 105 4.30 × 105 5.90 × 105 2.12 × 105 2.63 × 105