Table 2 In vitro tumor cell binding characteristics: determination of dissociation constants (Kd) in nM and apparent number of binding sites per cell (Bmax) of the 68Ga-labeled BN peptides (n = 3-5 experiments; mean values ± SD; Bmax SD < 15%)
| Â | 68Ga-DOTA-Ahx-BN | 68Ga-DOTA-Abz-BN | 68Ga-DOTA-ALA-BN | ||||
|---|---|---|---|---|---|---|---|
Cyclen | Tris- | Cyclen | Tris- | Cyclen | Tris- | ||
MDA | K d | 12.73 ± 2.94 | 11.60 ± 3.05 | 11.81 ± 2.11 | 13.10 ± 1.29 | 15.14 ± 3.12 | 14.68 ± 2.24 |
B max | 5.13 × 105 | 5.27 × 105 | 6.09 × 105 | 5.89 × 105 | 1.02 × 105 | 1.12 × 105 | |
MCF7 | K d | 9.80 ± 1.64 | 9.32 ± 1.50 | 10.75 ± 1.85 | 10.98 ± 1.10 | 13.69 ± 2.32 | 12.30 ± 1.94 |
B max | 6.90 × 105 | 6.22 × 105 | 5.12 × 105 | 4.86 × 105 | 2.11 × 105 | 1.05 × 105 | |
T47D | K d | 15.28 ± 3.42 | 14.51 ± 2.40 | 15.04 ± 3.24 | 13.82 ± 2.66 | 15.56 ± 3.47 | 16.0 ± 4.01 |
B max | 4.75 × 105 | 4.91 × 105 | 1.17 × 105 | 1.0 × 105 | 9.90 × 104 | 9.68 × 104 | |
PC3 | K d | 9.89 ± 1.56 | 10.45 ± 1.71 | 12.52 ± 2.09 | 11.24 ± 2.17 | 9.70 ± 2.01 | 13.49 ± 2.53 |
B max | 5.29 × 105 | 4.88 × 105 | 4.30 × 105 | 5.90 × 105 | 2.12 × 105 | 2.63 × 105 | |