Fig. 8

Western blot analyses of different protein expression such as PCNA, GRPR, p-p38, HIF-1α, p-53 in MDA-MB-231 cells treated with BNS, BN3, and BN4 at the indicated concentration. β-actin was used as the loading control. a Photomicrographs of immunohistochemical analyses of tumor sections, for expression of Ki-67 (cell proliferation marker) and CD-31 (angiogenesis marker) after BN4 treatment on tumor-bearing mice. b Results are representative of three independent experiments. The level of protein expression was found to increase gradually in a dose-dependent manner