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EJNMMI Research
Fig. 4 | EJNMMI Research

Fig. 4

From: Discovery and characterization of a high-affinity and high-specificity peptide ligand LXY30 for in vivo targeting of α3 integrin-expressing human tumors

Fig. 4

Integrin ligand LXY30 targets the conjugated SA-Cy5.5 dye to the subcutaneous and orthotopic xenograft tumors of α3β1 integrin-expressing U-87MG cells. U-87MG cells were stably transfected with luciferase and engrafted in the nude mice to generate both subcutaneous and orthotopic xenograft tumors. The mice were subjected to in vivo (a) and ex vivo (b) bioluminescence imaging (BLI) and optical NIRF imaging after LXY30-biotin/SA-Cy5.5 was injected. Both imaging approaches confirmed the uptake of LXY30-biotin/SA-Cy5.5 in the orthotopic xenograft tumors as well as the subcutaneous xenograft tumors of U-87MG cells. c The quantification of NIRF images indicated that both subcutaneous and orthotopic xenografts had higher uptakes of LXY30-biotin/SA-Cy5.5 compared to that of the normal brain. d Light microscopy of H&E staining and fluorescence microscopy of frozen sections of orthotopic and subcutaneous U-87MG xenograft tumors. LXY30-biotin/SA-Cy5.5 only accumulated in the subcutaneous (lower panel) and orthotopic U-87MG xenograft tumor cells, but not in adjacent normal tissue in the brain (upper panel). OT orthotopic tumor, NB normal brain. Scale bar: 100 μm

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