Skip to main content
EJNMMI Research
Fig. 3 | EJNMMI Research

Fig. 3

From: Discovery and characterization of a high-affinity and high-specificity peptide ligand LXY30 for in vivo targeting of α3 integrin-expressing human tumors

Fig. 3

Microscopy analysis of LXY30 on U-87MG cells and optical images of LXY30 in subcutaneous α3β1 integrin-expressing glioblastoma U-87MG tumors. U-87MG cells were incubated with LXY30-FITC or S-LXY30-FITC and then spun down onto slides for visualization by immunofluorescence microscopy. LXY30-FITC, but not S-LXY30-FITC, binds to the α3β1 integrin on the surface of glioblastoma U-87MG cells within 30 min of incubation. DAPI (diamidino-2-phenylindole) was used for the nuclear staining (blue). Scale bar: 50 μm (a). LXY30 also indicated accumulation inside U-87MG when cells were incubated for 2 h with LXY30-biotin conjugated with streptavidin (SA)-Alexa 488 under a confocal fluorescence microscope. The G6 peptide (GGGGGG) was used as negative control. Scale bar: 10 μm (b). LXY1-biotin/SA-Cy5.5 and LXY30-biotin/SA-Cy5.5 were injected into nude mice bearing subcutaneous U-87MG xenograft tumors. The mean fluorescence intensity of LXY30 in the region of interest at each xenograft tumor was quantified and compared to those of LXY1 and SA-Cy5.5 dye alone. LXY30 delivers more SA-Cy5.5 dye to the xenograft tumor compared to that of LXY1 (c, d)

Back to article page