Figure 1
Expression of NT-R by in vitro receptor autoradiography. NT-R expressed in a ductal pancreatic carcinoma: primary (A-C) and liver metastasis (D-F). As negative control, a NT-R-negative liver metastasis is also shown (G-I). (A), (D), (G) Hematoxylin-eosin-stained sections showing tumor tissue (Tu) and the adjacent pancreas (P) or liver (L), respectively. Bars = 1 mm. (B), (E) Autoradiograms showing total binding of 125I-[Tyr3]-neurotensin. High density of NT-R is found in the tumors but not in the pancreas or liver. (H) Autoradiogram showing total binding of 125I-neurotensin. This metastasis lacks NT-R. (C), (F), (I) Autoradiograms showing non-specific binding of 125I-[Tyr3]-neurotensin (in the presence of 100 nM neurotensin).