β2-adrenergic stress evaluation of coronary endothelial-dependent vasodilator function in mice using 11C-acetate micro-PET imaging of myocardial blood flow and oxidative metabolism

EJNMMI Research

Table 2 Hemodynamic response to NE- and SB-stress

Group	Stressor dose (μg/kg/min)	Mouse weight (g)	Rest			Stress
Group	Stressor dose (μg/kg/min)	Mouse weight (g)	Heart rate (bpm)	SBP (mmHg)	RPP (bpm∙mmHg)	Heart rate (bpm)	SBP (mmHg)	RPP (bpm∙mmHg)
Normal	NE 3.2	25 ± 3	409 ± 29	74 ± 12	30,018 ± 4,699	402 ± 25	89 ± 11	35,639 ± 4,557
	SB 0.2	27 ± 1	423 ± 21	88 ± 12	37,430 ± 5,850	433 ± 20	87 ± 14	37,593 ± 6,140
	SB 1.0	26 ± 1	432 ± 13	87 ± 13	37,729 ± 6,275	434 ± 11	85 ± 14	36,884 ± 6,587
	PHE + NE 3.2	25 ± 3	435 ± 32	95 ± 17	40,967 ± 6,151	476 ± 36*	77 ± 17	36,390 ± 7,695
eNOS knockout	NE 3.2	27 ± 6	355 ± 41^,*	107 ± 19^,*	38,516 ± 11,584	373 ± 42^,*	114 ± 17^,*	43,018 ± 11,214
NOS inhibition	NE 3.2	28 ± 3	454 ± 22*	79 ± 20	36,024 ± 10,195	453 ± 24*	85 ± 22	38,511 ± 10,921
	SB 0.2	31 ± 3	404 ± 44	90 ± 10	36,877 ± 5,278	409 ± 43	89 ± 8	37,021 ± 5,305
	SB 1.0	30 ± 2	408 ± 32	91 ± 11	37,247 ± 5,613	406 ± 30	91 ± 12	37,072 ± 6,122

bpm, heart beats per minute; SBP, systolic blood pressure; RPP, heart rate × systolic blood pressure product; eNOS, endothelial nitric oxide synthase; NE, norepinephrine; SB, salbutamol. NOS inhibition = L-NAME pretreated. Values are mean ± standard deviation. *p < 0.05 vs. normal NE. **p < 0.05 vs. NOS-inhibition NE.