Results from in vivo spectra in the study of gender influence in a glioblastoma rat model. Upper panel depicts both examples of T2W images and the selected region for acquiring the spectrum in a rat with (A) and without (B) tumour and the corresponding fitted spectra. (C) The percentage of variation in the concentration of several metabolites detected in tumour related to sham animals in both sexes. Unpaired t test analysis was performed on the data by comparing both genders in the same area. Statistically significant differences are indicated in the columns, where *P < 0.05, ***P < 0.001 and ****P < 0.0001. Ins, myo-inositol; GPC + PCH, glycerophosphorylcholine + phosphocholine; PCr + Cr, phosphocreatine + creatine; Tau, taurine; NAA, N-acetylaspartic acid.