Optimising the radiolabelling properties of technetium tricarbonyl and His-tagged proteins

EJNMMI Research

Table 2 Time taken to reach 95% LE of four His-tagged proteins at varying [Na+]

His-tagged protein	0.25 M [Na+]	0.44 M [Na+]	0.63 M [Na+]
rCR2	-	N/A	71.1 ± 3.8 min
α-CD33 scFv	N/A^a	106.8 ± 2 min	68.9 ± 1.2 min
α-PMSA scFv	N/A^b	51.5 ± 14 min	34.6 ± 6.4 min
α-VCAM-1 scFv	N/A^c	110.8 ± 5.1 min	73.2 ± 3.8 min

^aLE for α-CD33 scFv at 75 min was approximately 51.3%; ^bLE for α-PMSA scFv at 75 min was approximately 65.8%; ^cLE for α-VCAM-1 scFv at 75 min was approximately 52.7%.