Lymphoscintigraphy of the rats and lymph node dissection in the neck. Lymphoscintigraphy of the rats (A, left). The nanoparticle solution was injected into the tongue and the left upper limb. Rat B was administered 7.4 MBq of PCSN probes in the tongue and the left upper limb; rat A was injected with a smaller volume (3.7 MBq) in the tongue. The larger injection volume (rat B) revealed an increased uptake in the neck (arrow), with a marked increase in uptake at the site of injection in rat B. A small semiconductor gamma camera clearly displayed the uptake in the neck with an acquisition time of 30 s (A, right). Lymph nodes were dissected from the neck of rat B (B, left). Distinct ICG fluorescent lymph nodes in the neck were observed in real time with anatomical resolution (B, right).