Figure 1

Quality control of [¹²⁵ I]SGMIB-MNT. (a) Phosphor image of the dried SDS-PAGE gel (Any kD™ Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad, CA, USA) of [¹²⁵I]SGMIB-MNT. The main band at about 75 kD standard band corresponds to full-sized [¹²⁵I]SGMIB-MNT (57%), while the minor band at about 150 kD (12%) is consistent with an MNT dimer. (b) [¹²⁵I]SGMIB-MNT and (c) [¹²⁵I]iodoEGF binding to A431 cells. (d) [¹²⁵I]SGMIB-MNT and (e) [¹²⁵I]iodoEGF binding to D247 MG cells. Bars represent standard error of mean.