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Table 1 Labelling results of conjugates with Eu 3+ and 111 In 3+

From: Straightforward thiol-mediated protein labelling with DTPA: Synthesis of a highly active 111In-annexin A5-DTPA tracer

Incubation method

111In3+labelling in %

Eu3+labelling in %

2.4-fold excess of linker 4

46.2

36.7

8-fold excess of linker 4

53.1

36.2

24-fold excess of linker 4

58.6

41.7

for comparison:

  

cys-anxA5 5

0.0

0.0

  1. In case of europium the yield was determined by measuring the fluorescence, in case of 111In3+ by radioactivity measurement. The numbers are percentages of labelled protein.