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EJNMMI Research
Figure 3 | EJNMMI Research

Figure 3

From: Straightforward thiol-mediated protein labelling with DTPA: Synthesis of a highly active 111In-annexin A5-DTPA tracer

Figure 3

HPLC chromatogram of the reaction between  N  -(2-aminoethyl) maleimide trifluoroacetate (2) and cyclic DTPA anhydride (1). The peaks represent unreacted, hydrolysed DTPA (peak a), linker 4 (peak b) and byproduct 3 (peak c). Because of the presence of two UV-absorbing maleimide groups, the concentration of byproduct 3 is given by half of the peak integral in relation to peak b. The resulting ratio of product 4 to byproduct 3 is approximately 80:20.

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