Binding of TS1, an anti-keratin 8 antibody, in small-cell lung cancer after 177Lu-DOTA-Tyr3-octreotate treatment: a histological study in xenografted mice

EJNMMI Research

Table 2 Level of TS1 staining in selected regions of primary bronchial small-cell carcinoma biopsies

Case	Image 1	Image 2	Image 3	Image 4	Image 5	Image 6	Image 7	Image 8	Image 9
1	17.9	45.2	44.1	12.3	8.6	41.8	31.6	-	-
2	44.9	19.0	16.1	-	-	-	-	-	-
3	8.0	5.5	-	-	-	-	-	-	-
4	4.7	5.1	-	-	-	-	-	-	-
5	31.0	22.9	430	32.9	68.7	58.9	71.0	60.8	49.5
6	9.9	15.7	3.3	4.4	-	-	-	-	-
7	81.7^a	11.6	9.0	11.2	13.1	14.4	-	-	-

The biopsies had approximately the same size 10-20 mm² but the number of areas with tumour cells and necrotic tissue varied. Between two to nine 320 × 250-μm areas with tumour cells, necrotic tissue, and high TS1 binding per case were analysed. The percentage (%) DAB-positive immunostaining for TS1 in seven cases of primary bronchial small-cell carcinomas was determined by use of ImageJ ImmunoRatio.^aContains many intact tumour cells.