Fig. 1

Effects of [⁶⁴Cu]CuCl₂ exposure on three-day old spheroids. A–D Representative microscope images of eleven-day old spheroids exposed to 0, 0.6, 1.1, and 2.8 MBq. Growth curves represented by the mean spheroid area (µm²) of U87, U373, T98G, and RA spheroids, respectively, as a function of the number of days in culture are also shown. Scale bar: 500 µm. Data are presented as mean values ± S.E.M. of 2 to 4 independent assays. *p < 0.05, ****p < 0.0001