Fig. 2

Panel A shows the percentage of cell death for both cell lines after treatment with either 0.04 µM or 0.2 µM cisplatin after either conventional dose-response analysis and after recovery, derived from Fig. 1A and B. Panel B shows the cell cycle distribution analysis after cisplatin exposure. Both cell lines were exposed for 4 h with low dose (0.04 µM) cisplatin which correlates with the IC1.7 value of both VU-SCC-1131 and VU-SCC-OE in the recovery experiments with cisplatin-free medium, and high dose (0.2 µM) which correlates with the IC14.7 of VU-SCC-1131 and the IC7.0 of VU-SCC-OE. Cell cycle distribution was assessed directly after exposure and after 72 and 96 h recovery in cisplatin-free medium allowing repair. C) analysis of median fluorescence intensity (MFI) of EdU in S-phase differs between cell lines. VU-SCC-1131 shows at low and high dose ~ 82% reduction of the MFI after 72 h recovery, while in VU-SCC-OE MFI drops much less after 72 h recovery