Figure 6

Visualization of the difference between K _m and K _s . The plots cover a substantial range of the parameters k ₂ and k ₃, assuming a fixed ratio K ₁ / k ₂ = 0.7 ml/ml (a rationale for fixing this ratio is given in the discussion above). Top left, K _m ; top right, V _r ; bottom left, absolute difference (K _m  - K _s ); and bottom right, fractional difference ((K _m  - K _s ) / K _m ). Parameters and their respective units: k ₂, k ₃ (1/min); V _r (ml/ml); and K _m , K _s (ml/min/100 ml). Moving along the line, V _r  ≈ 0.55 ml/ml between K _m  = 1 and 4 ml/min/100 ml corresponds approximately to the experimental data of Figure 2A.