[18F]ROStrace detects oxidative stress in vivo and predicts progression of Alzheimer’s disease pathology in APP/PS1 mice

Background Oxidative stress is implicated in the pathogenesis of the most common neurodegenerative diseases, such as Alzheimer’s disease (AD). However, tracking oxidative stress in the brain has proven difficult and impeded its use as a biomarker. Herein, we investigate the utility of a novel positron emission tomography (PET) tracer, [18F]ROStrace, as a biomarker of oxidative stress throughout the course of AD in the well-established APP/PS1 double-mutant mouse model. PET imaging studies were conducted in wild-type (WT) and APP/PS1 mice at 3 different time points, representing early (5 mo.), middle (10 mo.), and advanced (16 mo.) life (n = 6–12, per sex). Semi-quantitation SUVRs of the plateau phase (40–60 min post-injection; SUVR40–60) of ten brain subregions were designated by the Mirrione atlas and analyzed by Pmod. Statistical parametric mapping (SPM) was used to distinguish brain regions with elevated ROS in APP/PS1 relative to WT in both sexes. The PET studies were validated by ex vivo autoradiography and immunofluorescence with the parent compound, dihydroethidium. Results [18F]ROStrace retention was increased in the APP/PS1 brain compared to age-matched controls by 10 mo. of age (p < 0.0001) and preceded the accumulation of oxidative damage in APP/PS1 neurons at 16 mo. (p < 0.005). [18F]ROStrace retention and oxidative damages were higher and occurred earlier in female APP/PS1 mice as measured by PET (p < 0.001), autoradiography, and immunohistochemistry (p < 0.05). [18F]ROStrace differences emerged midlife, temporally and spatially correlating with increased Aβ burden (r2 = 0.30; p = 0.0016), which was also greatest in the female brain (p < 0.001). Conclusions [18F]ROStrace identifies increased oxidative stress and neuroinflammation in APP/PS1 female mice, concurrent with increased amyloid burden midlife. Differences in oxidative stress during this crucial time may partially explain the sexual dimorphism in AD. [18F]ROStrace may provide a long-awaited tool to stratify at-risk patients who may benefit from antioxidant therapy prior to irreparable neurodegeneration. Supplementary Information The online version contains supplementary material available at 10.1186/s13550-022-00914-x.

those are women [1]. In the absence of preventative therapeutics or a cure, the number of AD sufferers will reach approximately 130 million worldwide by 2050 [2].
AD pathogenesis is estimated to begin decades before symptoms emerge [3,4]. Biomarkers that track the early pathogenic changes may provide an optimal therapeutic window by identifying those at risk of AD before irreversible brain damage and cognitive impairment occurs. Evidence from preclinical models and atrisk patients suggests the earliest biochemical changes in the AD brain involve glucose hypometabolism and reduced oxygen flow [5,6], oxidative stress [7][8][9][10][11][12], inflammation [13], and aberrant processing of amyloid precursor protein (APP) and tau [14][15][16][17]. Interestingly, each of these key changes has been linked to mitochondrial dysfunction and the production of reactive oxygen species (ROS) [18,19]. Mitochondrial function begins to decline during middle age [20], which may underlie the maternally inherited pattern of glucose hypometabolism in the at-risk AD brain [6,19,21,22]. Impaired mitochondria use ROS to alert the cellular environment of impending bioenergetic stress [23]. ROS refers to a family of partially reduced oxygen species, such as O 2 •− , HO • , H 2 O 2 , NO, and ONOO − , that possess highly reactive properties. ROS directly activate microglia, the resident macrophages of the brain. Activated microglia in turn produce more ROS via NADPH oxidase (NOX) and nitric oxide synthase (NOS) [24,25]. When the resulting rise in ROS production overwhelms the modest level of endogenous antioxidants in the aging brain, oxidative stress ensues. Oxidative stress modifies the enzymes responsible for pathogenic processing of APP and tau, leading to Aβ and tau accumulation [18]. Aβ and tau, along with oxidized lipids and DNA, can shift microglia into a chronic, ROS-generating phenotype [23,[25][26][27][28][29][30][31][32], thereby propelling the at-risk AD brain into a pro-inflammatory state that is neurotoxic. The primary ROS produced by mitochondria and activated microglia is superoxide (O 2 •− ). Thus, O 2 •− provides a signal of mitochondrial and immune (mito-immune) stress, which may be used to track neuroinflammation throughout the course of AD.
Our group has pioneered the development of positron emission tomography (PET) radiotracers for imaging oxidative stress in vivo [33,34]. The parent compound for radiotracer development is dihydroethidium (DHE), which is primarily oxidized by O 2 •− to ethidium and trapped in tissues [35]. Prior validation of [ 18 F]ROStrace demonstrates that it behaves similarly to DHE. Importantly, only the neutral species of [ 18 F]ROStrace crosses the blood brain barrier. Once in the brain, if [ 18 F]ROStrace is oxidized, [ 18 F]ox-ROStrace becomes trapped (Fig. 1a). This trapping mechanism results in increased [ 18 F]ROStrace retention in conditions of increased oxidative stress and neuroinflammation [34].
The two major goals of the current study were to determine if [ 18 F]ROStrace is sensitive enough (1) to detect increased oxidative stress in a preclinical model of AD and (2) to predict the accelerated AD pathology in the female brain. Increased amyloid beta (Aβ) is reported to be the earliest neuropathological change in the at-risk AD brain, and the presence of Aβ is currently required for AD diagnosis [36,37]. We therefore analyzed the relationship of [ 18 F]ROStrace and Aβ in the well-characterized, double-transgenic APP SWE /PS1dE9 (APP/PS1) mouse model. Much like AD patients, Aβ plaques, neuroinflammation, and cognitive defects are more prevalent in female compared to male APP/PS1 mice [38][39][40]. Due to the established role of oxidative stress in Aβ pathology, we hypothesized that [ 18 F]ROStrace retention would correlate with increased Aβ burden in female APP/PS1 mice. To test this hypothesis, a series of PET imaging studies were conducted in wild-type (WT) and APP/PS1 mice of both sexes at 3 different time points, representing early (5 mo.), middle (10 mo.), and advanced (16 mo.) life. The results of our study show that [ 18 F]ROStrace is increased in the APP/PS1 brain compared to WT. Further, [ 18 F] ROStrace retention is higher and occurs earlier in female APP/PS1 mice. Thus, female AD mice are more prone to oxidative stress than males, and this difference emerges with increased Aβ burden midlife. Our results suggest that differences in oxidative stress during this crucial time may partially explain the sexual dimorphism in AD.

[ 18 F]ROStrace retention is increased in APP/PS1 mice in vivo and reveals a female sex bias
Oxidative stress is a driver of AD pathology in APP/ PS1 mice [41,42]. To determine the sensitivity of [ 18 F] ROStrace to detect increased oxidative stress during the course of AD, a series of micro-PET imaging studies were performed in WT and APP/PS1 mice at 5 mo., 10 mo., and 16 mo. of age (Additional file 1: Fig. S1). Due to the lack of blood sample collection to perform absolute quantitation on [ 18 F]ROStrace PET images and differences in body weight between female and male ( Fig. 1b), we utilized a semiquantitative approach based on normalization to a reference region. However, since ROS produced by mitochondria or activated microglia could be throughout the brain, there is no true reference region for quantitating [ 18 F]ROStrace images. Studies of TSPO neuroinflammatory PET imaging agents have suggested using a pseudo-reference region, which is a region of interest (ROI) that has no difference in standardized uptake values (SUVs) among the study groups, to increase the sensitivity of the PET image analysis [43,44]. In the current study, the periaqueductal gray (PAG) was the ROI showing no statistical differences in the [ 18 F] ROStrace SUVs among WT and APP/PS1 in both genders at all ages (Fig. 1c). Therefore, this ROI was selected as the pseudo-reference region for [ 18 F]ROStrace SUV ratio (SUVR) measurement in this preclinical AD study.
In order to understand the effect of aging on retention of [ 18 F]ROStrace in brain tissue, SUVR 40-60 measurements were compared within female and male WT and APP/PS1 mice over time using a two-way ANOVA. The statistical comparisons show a decrease in several regions in male WT mice from 5 to 16 mo., but [ 18 F] ROStrace remained stable in most regions of the WT female brain over time (Additional file 1: Table S1). Conversely, [ 18 F]ROStrace retention increased over time in mice with APP/PS1 mutations, regardless of sex. From 5 to 16 mo., [ 18 F]ROStrace retention increased in the hippocampus (p = 0.0055), striatum (p = 0.0023), cortex (p = 0.0050), hypothalamus (p = 0.0204), and amygdala (p = 0.0157) of APP/PS1 females, but only the hippocampus (p = 0.0018) and cortex (p = 0.0296) of APP/PS1 males.

[ 18 F]ROStrace retention correlates with APP/PS1 amyloid burden
ROS induce Aβ-generating β-and γ-secretases [45], while inhibiting Aβ-clearing enzymes [46]. The resulting Aβ aggregates are associated with activated microglia and oxidative damage in the AD brain. Therefore, we hypothesized that [ 18 F]ROStrace levels would correlate with Aβ burden in APP/PS1 mice in a region-specific manner. To test this hypothesis, statistical parametric mapping (SPM) was first used to distinguish brain regions with elevated ROS in APP/PS1 relative to WT in both sexes (Fig. 4a). The SPM results show elevation of [ 18 F]ROStrace retention in APP/PS1 female mice at 10 mo. of age in hippocampus and cortex. By 16 mo., [ 18 F]ROStrace levels were augmented and extended to broader areas of both regions (Fig. 4a). In APP/PS1 males, higher [ 18 F] ROStrace was also observed in the hippocampus and cortex, but only at the most advanced age.
Next, the brains from 5-, 10-, and 16-mo.-old APP/ PS1 mice were processed with anti-amyloid and glial   PET images collected from both sexes of WT and APP/PS1 mice at 5 mo., 10 mo., and 16 mo. Brain subregions derived from Mirrione mouse brain atlas. Ctx: cortex, OB; olfactory bulb, BFS: basal forebrain septum, Hip: hippocampus, HyTh: hypothalamus, SC: superior colliculi, IC: inferior colliculi, Cb: cerebellum, BS: brain stem, Th: thalamus, PAG: periaqueductal gray, and Mb: midbrain fibrillary acidic protein (GFAP) antibodies. During proinflammatory conditions like trauma and amyloidosis, astrocytes assist in neuronal repair by creating a barrier to confine toxic elements and pave the way for microglial phagocytosis [47]. Our co-staining results confirm that amyloid plaques were closely surrounded by GFAP-positive astrocytes in the APP/PS1 brain. The representative images in Additional file 1: Fig. S2 show that very few Aβ aggregates were visible at 5 mo. of age in either sex, and these were predominately found in the olfactory bulb and the cortex. Female APP/PS1 mice also had sparse, small Aβ aggregates in the cerebellum at 5 mo. By 10 mo. of age, Aβ aggregates were detected throughout all four brain regions (olfactory bulb, cortex, hippocampus, and cerebellum), but were most prevalent in the cortex and hippocampus. A similar trend of Aβ distribution was seen at 16 mo.
To further investigate the relationship between regional amyloid burden and [ 18 F]ROStrace, low magnification images of sagittal brain sections were acquired from APP/ PS1 mice in each age group and the number of amyloid aggregates quantified in 4 key regions (Fig. 4b, Additional file 1: Fig. S2). The results show that the cortex is not only the first brain region where significant amyloid plaques appeared (p < 0.0001 for cortex vs. olfactory bulb, hippocampus, and cerebellum at 5 mo.), but also the region that bore the heaviest amyloid burden with increasing age (Fig. 4b, c). Amyloid burden in the cortex was higher Quantification of amyloid burden in 4 different brain regions of the APP/PS1 female (red) and male (blue) mouse brain. 5 mo. old APP/PS1 group: n = 9 for male, n = 5 for female, 10 mo. old APP/PS1 group: n = 4 per gender. 16 mo. old APP/ PS1 group: n = 8 for male, n = 5 for female. The statistical significance calculated by two-way ANOVA. *p < 0.05, **p < 0.005. ***p < 0.0001, relative to olfactory bulb, hippocampus, and cerebellum at 5-mo.-old APP/PS1 mouse. Values represent mean ± SEM. c Images of the cortex region of APP/PS1 mice 5-16 mo. of age stained with anti-GFAP (red), anti-beta amyloid 1-42 antibodies (green) and Hoechst (blue). Scale bar = 150 µm. d Average size of amyloid plaques in the cortex region of brains collected from 16-mo.-old APP/PS1 mice, n = 8 for male, n = 5 for female. The statistical significance of p value calculated by unpaired t test. Values represent mean ± SEM. *p < 0.05 e Linear regression analysis of cortical amyloid burden (n/mm 2 ) and [ 18 F]ROStrace SUVR  in APP/PS1 females than in age-matched males, which became significant at 10 mo. (Fig. 4b). The size of amyloid plaques was also largest in the cortex of APP/PS1 female mice at 16 mo. (Fig. 4d). These results confirm previous reports of increased Aβ burden in APP/PS1 females [38,39]. Interestingly, the cortex was also the first region to show increased [ 18 F]ROStrace levels in both sexes of APP/PS1 mice, and [ 18 F]ROStrace remained consistently higher in the APP/PS1 female vs. male cortex over time.
To evaluate the significance of this association, linear regression analysis was performed for amyloid burden versus [ 18 F]ROStrace SUVR  in the cortex of APP/ PS1 mice, which revealed a positive correlation between amyloid burden and [ 18 F]ROStrace (p = 0.0003, R 2 = 0.36; Fig. 4e). Collectively, these results highlight the sensitivity of [ 18 F]ROStrace to predict AD progression.

Validation of increased oxidative stress in the APP/PS1 brain
We further characterized the oxidative stress detected by [ 18 F]ROStrace in vivo by analyzing the same brains with established fluorescent detection methods. Following the PET scan, mice were injected with the parent compound, DHE (5 mg/kg, i.p.) and euthanized 30 min later. To estimate the cellular source of the [ 18 F]ROStrace signal, colocalization between DHE and three major brain cell types was then determined using cell-type specific markers for neurons (NeuN), microglia (IBA-1), and astrocytes (GFAP) (Fig. 5). The fluorescent images revealed that DHE was localized to the cytoplasm of neurons in the APP/PS1 brain only (Fig. 5a, b), which may represent Aβ-induced mitochondrial ROS [12,48]. DHE was also frequently found in aggregated microglia in the APP/ PS1 brain. These aggregated microglia surrounded amyloid plaques and had an amoeboid shape, with a large cell body and relatively few, short processes, resembling an activated morphology (Fig. 5d). In contrast, DHE was rarely detected in the microglia of age-matched WT brain (Fig. 5c). Lastly, DHE signal showed no detectable colocalization with GFAP, indicating that astrocytes have minimal involvement in the ROS signal detected by DHE (Fig. 5e, f ), and presumably [ 18 F]ROStrace, in both WT and APP/PS1 animals.
One of the key mediators of ROS-induced neurotoxicity in AD is peroxynitrite, (ONOO − ) [49,50]. ONOO − is a powerful oxidant produced by O 2 − and nitric oxide ( • NO) that permanently alters proteins via nitration of tyrosine residues. To determine the relationship between O 2 •− detected by [ 18 F]ROStrace and downstream oxidative damage, we quantified levels of 3-nitrotryosine (3NT) in age-matched WT and APP/PS1 mouse brains using IHC. A gradual elevation of 3NT was observed in the cortex of aging APP/PS1 mouse brain (Additional file 1: Fig. S3), which only became significant at 16 mo. of age (Fig. 6a). As expected, APP/PS1 females suffered the most extensive oxidative damage, evidenced by the highest number of 3NT-positive cells (Fig. 6b).

Discussion
The preclinical studies described herein suggest that oxidative stress may contribute to the increased risk of AD in women. Using the novel superoxide sensitive PET tracer, [ 18 F]ROStrace, we detected increased oxidative stress in the female AD brain in vivo, which coincided with increased Aβ and preceded the accumulation of neuronal damage. Increased Aβ is one of the earliest neuropathological changes in the at-risk AD brain and is required for AD diagnosis [36,37]. However, the disconnect between Aβ and cognitive performance presents a major limitation and suggests additional biomarkers are needed to predict the progression of neurodegeneration and gauge therapeutic efficacy throughout the course of disease [51]. Our results suggest [ 18 F]ROStrace is a promising tool to bridge this gap.
Mitochondrial dysfunction and microglial activation occur early in the neurodegeneration process, leading to a collective increase in ROS production and oxidative stress that continues through the course of AD. Markers of oxidative damage have been reported in mild cognitive impairment and early AD, but longitudinal analysis has thus far been limited to biofluids. Herein, we confirm the early and persistent role of oxidative stress in the brain using [ 18 F]ROStrace PET. We found that the fluorescent parent compound of [ 18 F]ROStrace, DHE, colocalized to neurons and microglia in the AD brain. These observations are in line with prior studies describing increased production of O 2 •− by neuronal mitochondria and activated microglia in response to Aβ [12,32,52,53]. In addition to O 2 •− , Aβ also induces • NO in both mitochondria and microglia [54,55] [56,57]. ONOO − permanently modifies proteins by nitrating tyrosine residues, which can thus be used as a molecular footprint of oxidative damage [58]. Using 3-nitrotyrosine, we found oxidative damage increased over time in the AD brain, as expected [49,50], but was highest in females. Notably, the increase in [ 18 F]ROStrace retention preceded oxidative damage in neurons, suggesting [ 18 F]ROStrace may serve as a predictive biomarker of downstream neuronal demise. The mito-immune shifts that occur in the female brain due to estrogen changes midlife are thought to be a major contributor to the increased risk of females to AD [59]. We selected 3 time points for [ 18 F]ROStrace imaging that, for female mice, represent key states which closely resemble the human condition during premenopause (5 mo.), perimenopause (10 mo.), and postmenopause (16 mo.) [60][61][62]. Interestingly, the only region with significantly different [ 18 F]ROStrace retention in WT females and males was the hypothalamus. The hypothalamus plays an important role of neuroendocrine function, especially in regulating estrogen in females [63][64][65][66]. The hypothalamic increase in [ 18 F]ROStrace retention in females began at 10 mo., which represents the midpoint of the proposed perimenopausal phase (7-12 mo.) in mice [61,62]. Perimenopause describes the midlife transition to reproductive senescence.
Perimenopause also represents a critical transition period in the aging female brain, requiring metabolic adaptation to the decline in estrogen-dependent bioenergetics [63]. Most women maintain the neurological resilience to transition through perimenopause without long-term consequences. However, for those at risk of AD, the neurological challenges involved in perimenopause may overwhelm the system and tip the scales toward neurodegeneration. Our results suggest that oxidative stress is a key indicator of this proposed tipping point.
Estrogen regulates mitochondrial biogenesis [67,68], endogenous antioxidants [69], and microglial activation [40,70]. Thus, in an environment of increased Aβ, such as the APP/PS1 brain, a decline in estrogen signaling would act as a second hit on mitochondrial function, leading to increased ROS production [71]. Increased mitochondrial ROS can drive the conversion of microglia into a pro-inflammatory phenotype by impairing oxidative phosphorylation, inducing mitochondrial fission, activating nuclear factor kappa B (NF-kB), and stimulating the metabolic switch to glycolysis [72]. Pro-inflammatory microglia amplify ROS production, which can become neurotoxic. Microglia in the female AD brain are predominately pro-inflammatory and less phagocytic than that of males, leading to increased amyloid burden and oxidative stress [40]. In preclinical models, oxidative stress induces every established pathological hallmark of AD [18]. Therefore, increased oxidative stress in the brain during midlife, when AD-related neurodegeneration is proposed to begin, may serve as a predictive biomarker of AD pathogenesis. Future studies are required to determine the underlying mechanisms responsible for increased oxidative stress in females, and whether beta-estradiol or other antioxidant therapy administered during this critical, early phase may prevent oxidative stress and slow AD progression in at-risk patients.

Conclusions
The current study demonstrates that the signal of [ 18 F] ROStrace is significantly increased in APP/PS1 mouse brain compared to age-matched WT controls. The high retention of [ 18 F]ROStrace also correlates with Aβ burden in cortex of aged APP/PS1 mouse brain. IHC analysis reveals that activated microglia and oxidative stress in neurons are the main contributors for the high [ 18 F]ROStrace signal in APP/PS1 mouse brain. Furthermore, [ 18 F] ROStrace retention is highest and occurs earlier in female APP/PS1 mice, peaking midlife during the perimenopausal phase. [ 18 F]ROStrace may also provide a long-awaited tool to stratify at-risk patients who may benefit from antioxidant therapy prior to irreparable neurodegeneration.

Animal models and experimental scheme
All animal experiments in this study were performed under protocols approved by the University of Pennsylvania Institutional Animal Care and Use Committee (IACUC). The double-transgenic APP SWE /PS1dE9 (APP/PS1) mice expressing Mo/HuAPP695swe (chimeric mouse and human amyloid precursor protein) and PS1dE9 (mutant human presenilin 1) and the litter-

Preparation of [ 18 F]ROStrace
The radiosynthesis of [ 18 F]ROStrace was prepared as previously described [34]. Briefly, [ 18 F]ROStrace was accomplished on all-in-One module (Trasis, Belgium) with full automation. The final product was diluted with 0.6 mL of ethanol, 0.1% ascorbic acid, and 6 mL normal saline and filtered by 0.2 µM nylon filter. The radiochemical yield was 4-20%, and the specific activity was 74 GBq/µmol.

Micro-PET imaging
PET imaging was performed on the β-Cube PET scanner (Molecubes, Ghent, Belgium). The body weight of each animal was measured before the PET scan (Fig. 1b). Animals were anesthetized with 1-2% isoflurane, a tail vein catheter was placed for PET tracer administration, and the animal was placed on the scanner bed. Dynamic scans of 0-60 min or 40-60 min post-injection were acquired after injection of 7.4-11.1 MBq of [ 18 F]ROStrace. After completion of the PET scan, the animal was transferred to the X-Cube CT scanner (Molecubes, Ghent, Belgium) and a general-purpose CT scan was acquired for anatomical reference and attenuation correction.

Micro-PET image analysis
All the [ 18 F]ROStrace micro-PET/CT imaging data were processed and analyzed by using Pmod software (version 3.7, PMOD Technologies Ltd., Zurich, Switzerland). Rigid body matching was manually performed on individual micro-CT images to co-register to the Mirrione mouse MR-T2 weighted brain template [73]. Then, the resulting transformation parameters were applied to the corresponding micro-PET image. Ten volumes of interest (VOIs) including cortex, thalamus, cerebellum, hypothalamus, brain stem, periaqueductal gray, striatum, hippocampus, amygdala, and midbrain were selected from the Mirrione atlas [73]. Due to the lack of the blood samples to perform absolute quantitation and a true reference region to semi-quantitate neuroinflammation images, a pseudo-reference region [44] is needed for [ 18 F] ROStrace neuroinflammation imaging evaluation. Therefore, the periaqueductal gray, with no significant difference in standardized uptake values (SUVs; Fig. 1b) among the different animal groups, was selected as the pseudoreference region for calculating the SUV ratio (SUVR) for each VOI. The 1-3 min SUVR and the 40-60 min SUVR (SUVR  were extracted from all the VOIs for the perfusion and the late phase comparison from the nineteen 16   parametric images between WT and APP/PS1 in each age group (5, 10, and 16 mo.) for both female and male.
Due to the small sample size of the studies, the SPM-Mouse analyses were evaluated by using a relative stringent threshold of a p value < 0.005 with uncorrected statistic and a voxel extent of 50.

Ex vivo autoradiography (ARG)
Mouse brains were collected immediately after [ 18 F] ROStrace micro-PET scan completion. The mouse brains were frozen and sectioned sagittally with a thickness of 30 µm on a cryostat (Leica Biosystems, Germany), and then, the tissue slides were air-dried for next step. The slides were exposed to phosphor plates (BAS 2040, GE Healthcare, Chicago, IL, USA) for 1 day, and the ARG images were digitized by the Typhoon FLA 7000 (West Avenue, Stamford, USA). The ARG images were analyzed by using Pmod software. The ROI of periaqueductal gray was manually delineated for further calculating the ratio of images relative to it.

Immunohistochemical (IHC) analysis
Collected brain tissues were embedded with Tissue-Tek optimal cutting temperature (Sakura, Japan) and frozen and then cut into 12 µm sagittal sections. The brain sections were fixed with 4% paraformaldehyde (PFA) at room temperature before staining and then rinsed with phosphate buffered saline (PBS). Then, 15 min of 0.1% Triton X-100 in PBS was applied to permeabilized tissues. After rinse with PBS, brain sections were blocked with 10% bovine serum albumin (BSA) for 1 h at room temperature. Selected slides were incubated with primary antibodies (Additional file 1: Table S2) overnight at 4 °C. The following day, after wash with 1% BSA in PBS, slides were incubated with corresponding secondary antibodies (Additional file 1: Table S2) for 1 h at room temperature. All slides were counterstained with Hoechst (1:2000, PBS, Thermo Fisher Scientific, H3570) and mounted with VectaShield Antifade Mounting Media (Vector Laboratories, 101098-042). Images were acquired by using Zeiss Axio Observer Microscope and Zeiss 710 Confocal Microscope (Germany). Panoramic brain tissue images were acquired by Keyence BZ-X800 Microscope (Osaka, Japan). For cortex amyloid burden and 3NT-positive cells quantification, images were uploaded onto Fiji software, amyloid plaques and 3NT-positive cells within the ROI (1.73 mm × 0.65 mm) were counted manually by using the cell counter plugin, and quantification results were scaled to n/mm 2 . The average plaque sizes of each Aβ plaque in brain were measured by Zen software (Zeiss, Germany).

Statistical analysis
All the statistical analyses were performed on GraphPad Prism software, version 7.02 (GraphPad Inc., San Diego, CA), and the results were presented as mean ± standard error (SEM) or mean ± standard deviation (SD) as noted. The statistical significance between different animal groups was determined either with one-way or two-way ANOVA following Sidak's or Turkey post hoc t test. A p value < 0.05 was considered as the threshold of statistically significant.
Additional file 1: Fig. S1. Schematic illustration of experimental scheme. Figure S2. Comparison of amyloid plaques spatial distribution in agematched APP/PS1 male and female mouse brains. Figure S3. Oxidative damage detected by 3NT in neurons over time in WT and APP/PS1 cortex. Table S1. Summary of regional SUVR 40-60 statistical analysis in age-and gender-matched APP/PS1 and WT mice at the age of 5, 10, and 16 mo. Table S2. Information of antibodies.