Fig. 2
From: Peptide-based PET imaging agent of tumor TIGIT expression
The TIGIT expression of 4T1 and A375 cells was demonstrated by western blot (a). Binding curve of 68 Ga-DOTA-DTBP-3 to TIGIT (b), showing a relative high affinity to murine TIGIT with the equilibrium dissociation constant KD was 84.21 nM. Cell uptake curve of 4T1 and A375 cells with different time points (15, 30, 60 and 120 min), 4T1 and A375 cells have a highest uptake of radiotracer with 8.41 ± 1.22% and 2.56 ± 0.19% at 0.5 h, respectively (c)