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EJNMMI Research
Fig. 1 | EJNMMI Research

Fig. 1

From: Development of a 64Cu-labeled CD4+ T cell targeting PET tracer: evaluation of CD4 specificity and its potential use in collagen-induced arthritis

Fig. 1

Development of [64Cu]Cu-NOTA-CD4: a CD4+ T cell targeting PET tracer. a Representative radio-HPLC chromatogram of [64Cu]Cu-NOTA-CD4. b Immunoreactivity of [64Cu]Cu-NOTA-CD4. c KD value of [64Cu]Cu-NOTA-CD4. d Merged and cropped SDS-PAGE of Coomassie staining and radiography of the same gel SDS-PAGE gel. Lane 1: Full-length anti-mouse CD4 antibody (IgG); Lane 2: Full-length anti-mouse CD4 antibody, reduced. Hc: Heavy chain. Lc: Light chain; Lane 3: [64Cu]Cu-NOTA-CD4; and Lane 4: [64Cu]Cu-NOTA-CD4, reduced. e The cyclic chelator NOTA was conjugated to F(ab)’2 fragments of R-anti-mouse CD4 antibodies and radiolabeled with Cu-64. Mice were i.v. injected with [64Cu]Cu-NOTA-CD4 and co-dose prior to PET/CT imaging

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