Fig. 4
Elevated [18F]ROStrace retention in the APP/PS1 brain maps with spatial distribution of amyloid deposits and positively correlates with increased amyloid burden in the cortex of aging APP/PS1 mouse brain. a Statistical parametric mapping (SPM) analysis showing significant differences (uncorrected p < 0.005 and an extent cluster threshold = 50) in [18F]ROStrace retention per sex in 5 mo. (top row), 10 mo. (middle row), and 16 mo. (bottom row) APP/PS1 mice versus WT. b Quantification of amyloid burden in 4 different brain regions of the APP/PS1 female (red) and male (blue) mouse brain. 5 mo. old APP/PS1 group: n = 9 for male, n = 5 for female, 10 mo. old APP/PS1 group: n = 4 per gender. 16 mo. old APP/PS1 group: n = 8 for male, n = 5 for female. The statistical significance calculated by two-way ANOVA. *p < 0.05, **p < 0.005. ***p < 0.0001, relative to olfactory bulb, hippocampus, and cerebellum at 5-mo.-old APP/PS1 mouse. Values represent mean ± SEM. c Images of the cortex region of APP/PS1 mice 5–16 mo. of age stained with anti-GFAP (red), anti-beta amyloid 1–42 antibodies (green) and Hoechst (blue). Scale bar = 150 µm. d Average size of amyloid plaques in the cortex region of brains collected from 16-mo.-old APP/PS1 mice, n = 8 for male, n = 5 for female. The statistical significance of p value calculated by unpaired t test. Values represent mean ± SEM. *p < 0.05 e Linear regression analysis of cortical amyloid burden (n/mm2) and [18F]ROStrace SUVR40-60