Fig. 3

Co-localization of CXCR4 and Sulfo-Cy5 signal in lesions at the squamocolumnar junction of IL1B mice. A Representative confocal images of stomach of injected IL1B mice of different grade of dysplasia and of one negative control (12 months old). Orange/yellow fluorescence indicated the co-localization of MK007 signal with CXCR4 Ab (white arrows) next to dysplastic areas at the SCJ. In some CXCR4 + cells, no MK007 uptake was observed (red arrows). The staining for CXCR4 was performed with anti-CXCR4 Ab conjugated with PE as described in the Methods section. Sulfo-Cy5 signal was detected by using Cy5.5 red channel. Scale bars represent 50 μm. B Quantification of CXCR4 expression and Sulfo-Cy5 signal in SCJ, squamous epithelium (Sq Ep) and glandular epithelium (Stomach): in the SCJ, the mean CXCR4 and Sulfo-Cy5 signal were 580.6 ± 105.2 and 465.8 ± 89.19, respectively. A much lower CXCR4 expression (211.9 ± 15.74) and Sulfo-Cy5 signal (272.8 ± 54.99) were detected in the glandular epithelium. One mouse presented a high CXCR4 expression in the inflamed squamous epithelium but without an increase of Sulfo-Cy5 signal. Data are represented as single plotted values and mean ± SEM (n = 5). C No Sulfo-Cy5 signal (mean positive area < 100 pixels) was detected in negative controls (n = 2). Signal quantification was expressed by calculating the mean positive area in pixels per each mouse and data are represented as single plotted values and mean ± SEM